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dc.contributor.authorSheahan, Patrick
dc.contributor.authorAhn, Chadwick N
dc.contributor.authorHarvey, Richard J
dc.contributor.authorWise, Sarah K
dc.contributor.authorMulligan, Ryan M
dc.contributor.authorLathers, Deanne M R
dc.contributor.authorSchlosser, Rodney J
dc.date.accessioned2012-02-01T10:42:54Z
dc.date.available2012-02-01T10:42:54Z
dc.date.issued2012-02-01T10:42:54Z
dc.identifier.citationJ Otolaryngol Head Neck Surg. 2010 Feb 1;39(1):45-51.en_GB
dc.identifier.issn1916-0216 (Print)en_GB
dc.identifier.pmid20122344en_GB
dc.identifier.urihttp://hdl.handle.net/10147/207772
dc.description.abstractINTRODUCTION: Chronic rhinosinusitis with nasal polyposis (CRSwNP) represents an eosinophilic T-helper 2 inflammatory response. Local production of IgE within nasal polyps (NPs) has been demonstrated, suggesting a role for local IgE in the pathogenesis of NP in atopic CRS patients. We hypothesized that local IgE specific to inhalant allergens may also play a role in the genesis of NP in nonatopic CRS patients. METHODS: Sinus and inferior turbinate tissue was obtained from nonatopic CRSwNP patients (n = 7), chronic rhinosinusitis without nasal polyps (CRSsNP) patients (n = 15), and healthy controls (n = 9) at the time of surgery. ImmunoCAP analysis (Phadia AB, Portage, MI) for 14 common inhalant antigens was performed on tissue homogenates to determine the antigen-specific response. RESULTS: Total IgE levels did not differ in sinus or turbinate tissue between CRSwNP, CRSsNP, or control patients. CRSwNP sinus tissue had higher levels of specific IgE for cockroach and plantain (p = .03) than other groups and elevated Alternaria IgE levels when compared with CRSsNP sinus tissue (p < .05). No significant differences were found for any of the other antigen-specific IgE levels. Fifty-seven percent of CRSwNP polyps demonstrated a polyclonal IgE response, whereas the other 43% had no demonstrable antigen-specific IgE. In contrast, only 17% of CRSsNP patients demonstrated a polyclonal response within sinus tissue, whereas 67% had no detectable antigen-specific IgE. There was no significant difference in levels of IgE in inferior turbinate tissue between the groups (p > .05). CONCLUSIONS: Localized mucosal IgE specific to common inhalant allergens appears to play a role in a subset of CRSwNP patients without evidence of systemic atopy.
dc.language.isoengen_GB
dc.subject.meshAllergensen_GB
dc.subject.meshEosinophils/metabolismen_GB
dc.subject.meshFemaleen_GB
dc.subject.meshHumansen_GB
dc.subject.meshImmunoglobulin E/*metabolismen_GB
dc.subject.meshImmunohistochemistryen_GB
dc.subject.meshMaleen_GB
dc.subject.meshMiddle Ageden_GB
dc.subject.meshNasal Polyps/*metabolismen_GB
dc.subject.meshRhinitis, Allergic, Perennial/*metabolismen_GB
dc.subject.meshTurbinates/metabolismen_GB
dc.titleLocal IgE production in nonatopic nasal polyposis.en_GB
dc.contributor.departmentDepartment of Otolaryngology-Head and Neck Surgery, South Infirmary Victoria, University Hospital, Cork, Ireland.en_GB
dc.identifier.journalJournal of otolaryngology - head & neck surgery = Le Journal, d'oto-rhino-laryngologie et de chirurgie cervico-facialeen_GB
dc.description.provinceMunster
html.description.abstractINTRODUCTION: Chronic rhinosinusitis with nasal polyposis (CRSwNP) represents an eosinophilic T-helper 2 inflammatory response. Local production of IgE within nasal polyps (NPs) has been demonstrated, suggesting a role for local IgE in the pathogenesis of NP in atopic CRS patients. We hypothesized that local IgE specific to inhalant allergens may also play a role in the genesis of NP in nonatopic CRS patients. METHODS: Sinus and inferior turbinate tissue was obtained from nonatopic CRSwNP patients (n = 7), chronic rhinosinusitis without nasal polyps (CRSsNP) patients (n = 15), and healthy controls (n = 9) at the time of surgery. ImmunoCAP analysis (Phadia AB, Portage, MI) for 14 common inhalant antigens was performed on tissue homogenates to determine the antigen-specific response. RESULTS: Total IgE levels did not differ in sinus or turbinate tissue between CRSwNP, CRSsNP, or control patients. CRSwNP sinus tissue had higher levels of specific IgE for cockroach and plantain (p = .03) than other groups and elevated Alternaria IgE levels when compared with CRSsNP sinus tissue (p < .05). No significant differences were found for any of the other antigen-specific IgE levels. Fifty-seven percent of CRSwNP polyps demonstrated a polyclonal IgE response, whereas the other 43% had no demonstrable antigen-specific IgE. In contrast, only 17% of CRSsNP patients demonstrated a polyclonal response within sinus tissue, whereas 67% had no detectable antigen-specific IgE. There was no significant difference in levels of IgE in inferior turbinate tissue between the groups (p > .05). CONCLUSIONS: Localized mucosal IgE specific to common inhalant allergens appears to play a role in a subset of CRSwNP patients without evidence of systemic atopy.


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