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dc.contributor.authorKevans, David
dc.contributor.authorFoley, Jane
dc.contributor.authorTenniswood, Martin
dc.contributor.authorSheahan, Kieran
dc.contributor.authorHyland, John
dc.contributor.authorO'Donoghue, Diarmuid
dc.contributor.authorMulcahy, Hugh
dc.contributor.authorO'Sullivan, Jacintha
dc.date.accessioned2012-02-01T10:31:38Z
dc.date.available2012-02-01T10:31:38Z
dc.date.issued2012-02-01T10:31:38Z
dc.identifier.citationCancer Epidemiol Biomarkers Prev. 2009 Feb;18(2):393-9. Epub 2009 Jan 20.en_GB
dc.identifier.issn1055-9965 (Print)en_GB
dc.identifier.issn1055-9965 (Linking)en_GB
dc.identifier.pmid19155441en_GB
dc.identifier.doi10.1158/1055-9965.EPI-08-0302en_GB
dc.identifier.urihttp://hdl.handle.net/10147/207569
dc.description.abstractThe role of clusterin in tumor growth and progression remains unclear. Overexpression of cytoplasmic clusterin has been studied in aggressive colon tumors; however, no correlation between clusterin expression and survival in colorectal cancer has been identified to date. We assessed levels of clusterin expression in a group of stage II colorectal cancer patients to assess its utility as a prognostic marker. The study included 251 patients with stage II colorectal cancer. Tissue microarrays were constructed and immunohistochemistry done and correlated with clinical features and long term outcome. Dual immunofluorescence and confocal microscopy were used with terminal deoxynucleotidyl-transferase-mediated dUTP nick-end labeling probes and clusterin antibody to assess the degree of co localization. Percentage epithelial cytoplasmic staining was higher in tumor compared with nonadjacent normal mucosa (P < 0.001). Within the stromal compartment, percentage cytoplamic staining and intensity was lower in tumor tissue compared with normal nonadjacent mucosa (P < or = 0.001). Survival was significantly associated with percentage epithelial cytoplasmic staining (P < 0.001), epithelial cytoplasmic staining intensity (P < 0.001), percentage stromal cytoplasmic staining (P = 0.002), and stromal cytoplasmic staining intensity (P < 0.001). Clusterin levels are associated with poor survival in stage II colorectal cancer.
dc.language.isoengen_GB
dc.subject.meshAdulten_GB
dc.subject.meshAgeden_GB
dc.subject.meshAged, 80 and overen_GB
dc.subject.meshChi-Square Distributionen_GB
dc.subject.meshClusterin/*metabolismen_GB
dc.subject.meshColorectal Neoplasms/*metabolism/pathologyen_GB
dc.subject.meshFemaleen_GB
dc.subject.meshHumansen_GB
dc.subject.meshImmunoenzyme Techniquesen_GB
dc.subject.meshIn Situ Nick-End Labelingen_GB
dc.subject.meshMaleen_GB
dc.subject.meshMicroarray Analysisen_GB
dc.subject.meshMiddle Ageden_GB
dc.subject.meshNeoplasm Stagingen_GB
dc.subject.meshPrognosisen_GB
dc.subject.meshReproducibility of Resultsen_GB
dc.subject.meshStatistics, Nonparametricen_GB
dc.subject.meshTumor Markers, Biological/*metabolismen_GB
dc.titleHigh clusterin expression correlates with a poor outcome in stage II colorectal cancers.en_GB
dc.contributor.departmentCentre for Colorectal Disease, St Vincent's University Hospital, Elm Park, Dublin, 4, Ireland.en_GB
dc.identifier.journalCancer epidemiology, biomarkers & prevention : a publication of the American, Association for Cancer Research, cosponsored by the American Society of, Preventive Oncologyen_GB
dc.description.provinceLeinster
html.description.abstractThe role of clusterin in tumor growth and progression remains unclear. Overexpression of cytoplasmic clusterin has been studied in aggressive colon tumors; however, no correlation between clusterin expression and survival in colorectal cancer has been identified to date. We assessed levels of clusterin expression in a group of stage II colorectal cancer patients to assess its utility as a prognostic marker. The study included 251 patients with stage II colorectal cancer. Tissue microarrays were constructed and immunohistochemistry done and correlated with clinical features and long term outcome. Dual immunofluorescence and confocal microscopy were used with terminal deoxynucleotidyl-transferase-mediated dUTP nick-end labeling probes and clusterin antibody to assess the degree of co localization. Percentage epithelial cytoplasmic staining was higher in tumor compared with nonadjacent normal mucosa (P < 0.001). Within the stromal compartment, percentage cytoplamic staining and intensity was lower in tumor tissue compared with normal nonadjacent mucosa (P < or = 0.001). Survival was significantly associated with percentage epithelial cytoplasmic staining (P < 0.001), epithelial cytoplasmic staining intensity (P < 0.001), percentage stromal cytoplasmic staining (P = 0.002), and stromal cytoplasmic staining intensity (P < 0.001). Clusterin levels are associated with poor survival in stage II colorectal cancer.


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