• Login
    View Item 
    •   Home
    • Hospital Research
    • Leinster
    • St. Vincent's University Hospital
    • View Item
    •   Home
    • Hospital Research
    • Leinster
    • St. Vincent's University Hospital
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Map of Submissions

    Home Page
    UlsterN
    4715
    UlsterS
    4715
    Connacht
    1603
    Munster
    48
    Leinster
    426

    Browse

    All of Lenus, The Irish Health RepositoryCommunitiesTitleAuthorsDate publishedSubjectsThis CollectionTitleAuthorsDate publishedSubjects

    My Account

    LoginRegister

    About

    About LenusDirectory of Open Access JournalsOpen Access Publishing GuideNational Health Library & Knowledge ServiceGuide to Publishers' PoliciesFAQsTerms and ConditionsVision StatementRIAN Pathways to Irish ResearchHSE position statement on Open AccessNational Open Research Forum (NORF)Zenodo (European Open Research repository)

    Statistics

    Display statistics

    Bacterial lipopolysaccharide promotes profibrotic activation of intestinal fibroblasts.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Burke, J P
    Cunningham, M F
    Watson, R W G
    Docherty, N G
    Coffey, J C
    O'Connell, P R
    Affiliation
    Department of Surgery, St Vincent's University Hospital, Dublin, Ireland.
    Issue Date
    2012-02-01T10:28:56Z
    MeSH
    Aged
    Aged, 80 and over
    Cells, Cultured
    Colonic Neoplasms/metabolism
    Connective Tissue Growth Factor/biosynthesis
    Fibroblasts/*drug effects/metabolism
    Humans
    I-kappa B Kinase/metabolism
    Lipopolysaccharides/*pharmacology
    Middle Aged
    NF-kappa B/metabolism
    Smad7 Protein/metabolism
    Toll-Like Receptor 4/*metabolism
    Transforming Growth Factor beta1/*pharmacology
    Wound Healing/*physiology
    Show allShow less
    
    Metadata
    Show full item record
    Citation
    Br J Surg. 2010 Jul;97(7):1126-34.
    Journal
    The British journal of surgery
    URI
    http://hdl.handle.net/10147/207477
    DOI
    10.1002/bjs.7045
    PubMed ID
    20632282
    Abstract
    BACKGROUND: Fibroblasts play a critical role in intestinal wound healing. Lipopolysaccharide (LPS) is a cell wall component of commensal gut bacteria. The effects of LPS on intestinal fibroblast activation were characterized. METHODS: Expression of the LPS receptor, toll-like receptor (TLR) 4, was assessed in cultured primary human intestinal fibroblasts using flow cytometry and confocal microscopy. Fibroblasts were treated with LPS and/or transforming growth factor (TGF) beta1. Nuclear factor kappaB (NFkappaB) pathway activation was assessed by inhibitory kappaBalpha (IkappaBalpha) degradation and NFkappaB promoter activity. Fibroblast contractility was measured using a fibroblast-populated collagen lattice. Smad-7, a negative regulator of TGF-beta1 signalling, and connective tissue growth factor (CTGF) expression were assessed using reverse transcriptase-polymerase chain reaction and western blot. The NFkappaB pathway was inhibited by IkappaBalpha transfection. RESULTS: TLR-4 was present on the surface of intestinal fibroblasts. LPS treatment of fibroblasts induced IkappaBalpha degradation, enhanced NFkappaB promoter activity and increased collagen contraction. Pretreatment with LPS (before TGF-beta1) significantly increased CTGF production relative to treatment with TGF-beta1 alone. LPS reduced whereas TGF-beta1 increased smad-7 expression. Transfection with an IkappaBalpha plasmid enhanced basal smad-7 expression. CONCLUSION: Intestinal fibroblasts express TLR-4 and respond to LPS by activating NFkappaB and inducing collagen contraction. LPS acts in concert with TGF-beta1 to induce CTGF. LPS reduces the expression of the TGF-beta1 inhibitor, smad-7.
    Language
    eng
    ISSN
    1365-2168 (Electronic)
    0007-1323 (Linking)
    ae974a485f413a2113503eed53cd6c53
    10.1002/bjs.7045
    Scopus Count
    Collections
    St. Vincent's University Hospital

    entitlement

    Related articles

    • Simvastatin impairs smad-3 phosphorylation and modulates transforming growth factor beta1-mediated activation of intestinal fibroblasts.
    • Authors: Burke JP, Watson RW, Murphy M, Docherty NG, Coffey JC, O'Connell PR
    • Issue date: 2009 May
    • Bacterial lipopolysaccharide promotes profibrotic activation of intestinal fibroblasts (Br J Surg 2010; 97: 1126-1134).
    • Authors: Bhogal RH, Sutaria R, Afford SC
    • Issue date: 2010 Nov
    • Transforming growth factor-beta 1 inhibits non-pathogenic Gram negative bacteria-induced NF-kappa B recruitment to the interleukin-6 gene promoter in intestinal epithelial cells through modulation of histone acetylation.
    • Authors: Haller D, Holt L, Kim SC, Schwabe RF, Sartor RB, Jobin C
    • Issue date: 2003 Jun 27
    • Effect of transforming growth factor-beta1 on expression of the connective tissue growth factor (CCN2/CTGF) gene in normal human gingival fibroblasts and periodontal ligament cells.
    • Authors: Takeuchi H, Kubota S, Murakashi E, Fukada T, Hashimoto S, Takigawa M, Numabe Y
    • Issue date: 2009 Apr
    • Transforming growth factor beta 1(TGF-beta1) down-regulates TNFalpha-induced RANTES production in rheumatoid synovial fibroblasts through NF-kappaB-mediated transcriptional repression.
    • Authors: Cho ML, Min SY, Chang SH, Kim KW, Heo SB, Lee SH, Park SH, Cho CS, Kim HY
    • Issue date: 2006 Jun 15
    National Health Library & Knowledge Service | Health Service Executive | Dr Steevens' Hospital | Dublin 8 | Ireland
    lenus@hse.ie | Tel +353 (1) 6352558
    DSpace software copyright © 2002-2017  DuraSpace
    Contact Us | Disclaimer
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.