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dc.contributor.authorCorcionivoschi, N
dc.contributor.authorClyne, M
dc.contributor.authorLyons, A
dc.contributor.authorElmi, A
dc.contributor.authorGundogdu, O
dc.contributor.authorWren, B W
dc.contributor.authorDorrell, N
dc.contributor.authorKarlyshev, A V
dc.contributor.authorBourke, B
dc.date.accessioned2012-02-01T10:24:21Z
dc.date.available2012-02-01T10:24:21Z
dc.date.issued2012-02-01T10:24:21Z
dc.identifier.citationInfect Immun. 2009 May;77(5):1959-67. Epub 2009 Mar 9.en_GB
dc.identifier.issn1098-5522 (Electronic)en_GB
dc.identifier.issn0019-9567 (Linking)en_GB
dc.identifier.pmid19273563en_GB
dc.identifier.doi10.1128/IAI.01239-08en_GB
dc.identifier.urihttp://hdl.handle.net/10147/207412
dc.description.abstractThe host cell environment can alter bacterial pathogenicity. We employed a combination of cellular and molecular techniques to study the expression of Campylobacter jejuni polysaccharides cocultured with HCT-8 epithelial cells. After two passages, the amount of membrane-bound high-molecular-weight polysaccharide was considerably reduced. Microarray profiling confirmed significant downregulation of capsular polysaccharide (CPS) locus genes. Experiments using conditioned media showed that sugar depletion occurred only when the bacterial and epithelial cells were cocultured. CPS depletion occurred when C. jejuni organisms were exposed to conditioned media from a different C. jejuni strain but not when exposed to conditioned media from other bacterial species. Proteinase K or heat treatment of conditioned media under coculture conditions abrogated the effect on the sugars, as did formaldehyde fixation and cycloheximide treatment of host cells or chloramphenicol treatment of the bacteria. However, sugar depletion was not affected in flagellar export (fliQ) and quorum-sensing (luxS) gene mutants. Passaged C. jejuni showed reduced invasiveness and increased serum sensitivity in vitro. C. jejuni alters its surface polysaccharides when cocultured with epithelial cells, suggesting the existence of a cross talk mechanism that modulates CPS expression during infection.
dc.language.isoengen_GB
dc.subject.meshCampylobacter jejuni/*physiologyen_GB
dc.subject.meshCell Lineen_GB
dc.subject.meshCoculture Techniquesen_GB
dc.subject.meshCulture Media, Conditioneden_GB
dc.subject.meshEpithelial Cells/*microbiologyen_GB
dc.subject.meshGene Expression Profilingen_GB
dc.subject.meshHumansen_GB
dc.subject.meshOligonucleotide Array Sequence Analysisen_GB
dc.subject.meshPolysaccharides, Bacterial/*biosynthesisen_GB
dc.titleCampylobacter jejuni cocultured with epithelial cells reduces surface capsular polysaccharide expression.en_GB
dc.contributor.departmentThe Children's Research Centre, Our Lady's Children's Hospital, Crumlin, Dublin, 12, Ireland.en_GB
dc.identifier.journalInfection and immunityen_GB
dc.description.provinceLeinster
html.description.abstractThe host cell environment can alter bacterial pathogenicity. We employed a combination of cellular and molecular techniques to study the expression of Campylobacter jejuni polysaccharides cocultured with HCT-8 epithelial cells. After two passages, the amount of membrane-bound high-molecular-weight polysaccharide was considerably reduced. Microarray profiling confirmed significant downregulation of capsular polysaccharide (CPS) locus genes. Experiments using conditioned media showed that sugar depletion occurred only when the bacterial and epithelial cells were cocultured. CPS depletion occurred when C. jejuni organisms were exposed to conditioned media from a different C. jejuni strain but not when exposed to conditioned media from other bacterial species. Proteinase K or heat treatment of conditioned media under coculture conditions abrogated the effect on the sugars, as did formaldehyde fixation and cycloheximide treatment of host cells or chloramphenicol treatment of the bacteria. However, sugar depletion was not affected in flagellar export (fliQ) and quorum-sensing (luxS) gene mutants. Passaged C. jejuni showed reduced invasiveness and increased serum sensitivity in vitro. C. jejuni alters its surface polysaccharides when cocultured with epithelial cells, suggesting the existence of a cross talk mechanism that modulates CPS expression during infection.


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