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    Evaluation of the efficacy of real-time polymerase chain reaction for the routine early detection of Pseudomonas aeruginosa in cystic fibrosis sputum and throat swab specimens.

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    Authors
    Logan, Catriona
    Habington, Adele
    Lennon, Grainne
    Cronin, Frank
    O'Sullivan, Niamh
    Affiliation
    Department of Microbiology, Our Lady's Children's Hospital, Crumlin, Dublin,, Ireland. catrionalogan@eircom.net
    Issue Date
    2012-02-01T10:23:57Z
    MeSH
    Adolescent
    Bacteriological Techniques
    Child
    Child, Preschool
    Culture Media
    Cystic Fibrosis/*microbiology
    DNA, Bacterial/analysis/isolation & purification
    Early Diagnosis
    Humans
    Infant
    Infant, Newborn
    Pharynx/*microbiology
    Polymerase Chain Reaction/*methods
    Pseudomonas Infections/*diagnosis/microbiology
    Pseudomonas aeruginosa/genetics/*isolation & purification
    Sensitivity and Specificity
    Specimen Handling/methods
    Sputum/*microbiology
    Young Adult
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    Citation
    Diagn Microbiol Infect Dis. 2010 Dec;68(4):358-65. Epub 2010 Sep 29.
    Journal
    Diagnostic microbiology and infectious disease
    URI
    http://hdl.handle.net/10147/207397
    DOI
    10.1016/j.diagmicrobio.2010.07.012
    PubMed ID
    20884156
    Abstract
    A longitudinal study of 2099 sputa and throat swabs received from 183 pediatric cystic fibrosis patients over a 29-month period was used to evaluate the efficacy of real-time polymerase chain reaction (PCR) for the early detection of Pseudomonas aeruginosa as compared to microbiologic culture. Real-time PCR resulted in an increased number of specimens identified as P. aeruginosa positive. The sensitivity of culture was 82% (373/453) and of PCR was 93% (420/453) when considering both positive culture and PCR results as true positives. Of the 80 specimens identified as PCR positive/culture negative for P. aeruginosa, the subsequent patient sample in 32.5% (26/80) of specimens concerned was identified as P. aeruginosa culture positive, suggesting that PCR has the potential to detect P. aeruginosa earlier than the microbiologic culture. Real-time PCR analysis found no evidence of the Liverpool and Manchester epidemic P. aeruginosa strains in the cohort examined. The findings of this study highlight the importance of specimen collection protocols to ensure that adequate samples are received at the laboratory for testing, thereby minimizing the potential for reporting of false-negative P. aeruginosa culture results.
    Language
    eng
    ISSN
    1879-0070 (Electronic)
    0732-8893 (Linking)
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.diagmicrobio.2010.07.012
    Scopus Count
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    Our Lady's Children's Hospital Crumlin

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