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dc.contributor.authorGriffin, Alison
dc.contributor.authorO'Shea, Paula
dc.contributor.authorFitzGerald, Roland
dc.contributor.authorO'Connor, Gerard
dc.contributor.authorTormey, William
dc.date.accessioned2012-02-01T10:01:56Z
dc.date.available2012-02-01T10:01:56Z
dc.date.issued2012-02-01T10:01:56Z
dc.identifier.citationAnn Clin Biochem. 2011 Jan;48(Pt 1):41-4. Epub 2010 Dec 1.en_GB
dc.identifier.issn1758-1001 (Electronic)en_GB
dc.identifier.issn0004-5632 (Linking)en_GB
dc.identifier.pmid21123298en_GB
dc.identifier.doi10.1258/acb.2010.010062en_GB
dc.identifier.urihttp://hdl.handle.net/10147/207206
dc.description.abstractINTRODUCTION: Normetanephrine and metanephrine are intermediate metabolites of noradrenaline and adrenaline metabolism. To assess whether normetanephrine and metanephrine analysis may aid in the diagnosis of Neuroblastoma, a reference interval for these metabolites must first be established. AIM: The overall aim of this study was to establish a paediatric age-related reference interval for the measurement of total fractionated metanephrines. METHODS: A total of 267 urine samples were analysed following acid hydrolysis. This releases the metanephrines from their sulphate-bound metabolites. The samples were analysed using reverse phase high-performance liquid chromatography with electro-chemical detection on a Gilson automated sequential trace enrichment of dialysate sample system. RESULTS: Data were analysed using Minitab Release version 14. Outliers were removed using the Dixon/Reed one-third rule. Partitioning of the age groups was achieved using Harris and Boyd's standard normal deviate test. Non-parametric analysis of the data was performed, followed by the establishment of the 2.5th and the 97.5th reference limits. CONCLUSIONS: The established reference intervals are described in Table 2.
dc.language.isoengen_GB
dc.subject.meshAge Factorsen_GB
dc.subject.meshChilden_GB
dc.subject.meshChild, Preschoolen_GB
dc.subject.meshChromatography, Reverse-Phaseen_GB
dc.subject.meshEpinephrine/metabolismen_GB
dc.subject.meshFemaleen_GB
dc.subject.meshHumansen_GB
dc.subject.meshInfanten_GB
dc.subject.meshMaleen_GB
dc.subject.meshMetanephrine/*urineen_GB
dc.subject.meshNeuroblastoma/*diagnosis/urineen_GB
dc.subject.meshNorepinephrine/metabolismen_GB
dc.subject.meshNormetanephrine/*urineen_GB
dc.subject.meshROC Curveen_GB
dc.subject.meshReference Valuesen_GB
dc.subject.meshStatistics, Nonparametricen_GB
dc.titleEstablishment of a paediatric age-related reference interval for the measurement of urinary total fractionated metanephrines.en_GB
dc.contributor.departmentDepartment of Chemical Pathology, Beaumont Hospital, Dublin 9, Ireland., alisongriffin@beaumont.ieen_GB
dc.identifier.journalAnnals of clinical biochemistryen_GB
dc.description.provinceLeinster
html.description.abstractINTRODUCTION: Normetanephrine and metanephrine are intermediate metabolites of noradrenaline and adrenaline metabolism. To assess whether normetanephrine and metanephrine analysis may aid in the diagnosis of Neuroblastoma, a reference interval for these metabolites must first be established. AIM: The overall aim of this study was to establish a paediatric age-related reference interval for the measurement of total fractionated metanephrines. METHODS: A total of 267 urine samples were analysed following acid hydrolysis. This releases the metanephrines from their sulphate-bound metabolites. The samples were analysed using reverse phase high-performance liquid chromatography with electro-chemical detection on a Gilson automated sequential trace enrichment of dialysate sample system. RESULTS: Data were analysed using Minitab Release version 14. Outliers were removed using the Dixon/Reed one-third rule. Partitioning of the age groups was achieved using Harris and Boyd's standard normal deviate test. Non-parametric analysis of the data was performed, followed by the establishment of the 2.5th and the 97.5th reference limits. CONCLUSIONS: The established reference intervals are described in Table 2.


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