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    Comparison of the EntericBio multiplex PCR system with routine culture for detection of bacterial enteric pathogens.

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    Authors
    O'Leary, James
    Corcoran, Daniel
    Lucey, Brigid
    Affiliation
    Department of Medical Microbiology, Cork University Hospital, Wilton, Cork, Ireland.
    Issue Date
    2009-11
    MeSH
    Bacteriological Techniques
    Campylobacter
    Enterobacteriaceae
    Humans
    Molecular Diagnostic Techniques
    Nucleic Acid Hybridization
    Polymerase Chain Reaction
    Predictive Value of Tests
    Reagent Kits, Diagnostic
    Sensitivity and Specificity
    Time Factors
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    Citation
    Comparison of the EntericBio multiplex PCR system with routine culture for detection of bacterial enteric pathogens. 2009, 47 (11):3449-53 J. Clin. Microbiol.
    Journal
    Journal of clinical microbiology
    URI
    http://hdl.handle.net/10147/200777
    DOI
    10.1128/JCM.01026-09
    PubMed ID
    19726596
    Additional Links
    http://jcm.asm.org/content/47/11/3449.full.pdf+html
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2772650/pdf/1026-09.pdf
    Abstract
    The EntericBio system uses a multiplex PCR assay for the simultaneous detection of Campylobacter spp., Salmonella enterica, Shigella spp., and Escherichia coli O157 from feces. It combines overnight broth enrichment with PCR amplification and detection by hybridization. An evaluation of this system was conducted by comparing the results obtained with the system with those obtained by routine culture, supplemented with alternative PCR detection methods. In a study of 773 samples, routine culture and the EntericBio system yielded 94.6 and 92.4% negative results, respectively. Forty-two samples had positive results by culture, and all of these were positive with the EntericBio system. This system detected an additional 17 positive samples (Campylobacter spp., n = 12; Shigella spp., n = 1; E. coli O157, n = 4), but the results for 5 samples (Campylobacter spp., n = 2; Shigella spp., n = 1; E. coli O157, n = 2) could not be confirmed. The target for Shigella spp. detected by the EntericBio system is the ipaH gene, and the molecular indication of the presence of Shigella spp. was investigated by sequence analysis, which confirmed that the ipaH gene was present in a Klebsiella pneumoniae isolate from the patient. The sensitivity, specificity, positive predictive value, and negative predictive value were 100%, 99.3%, 91.5%, and 100%, respectively. Turnaround times were significantly reduced with the EntericBio system, and a result was available between 24 and 32 h after receipt of the sample in the laboratory. In addition, the amount of laboratory waste was significantly reduced by use of this system. In summary, the EntericBio system proved convenient to use, more sensitive than the conventional culture used in this study, and highly specific; and it generated results significantly faster than routine culture for the pathogens tested.
    Item Type
    Article
    Language
    en
    ISSN
    1098-660X
    ae974a485f413a2113503eed53cd6c53
    10.1128/JCM.01026-09
    Scopus Count
    Collections
    Cork University Hospital

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