A molecular analysis of fecal and mucosal bacterial communities in irritable bowel syndrome.
Affiliation
Department of Medicine, Alimentary Pharmabiotic Centre, Cork University Hospital, Cork, Ireland.Issue Date
2010-02MeSH
AdultAged
Bacteria
Biopsy
Colon
DNA, Bacterial
Feces
Female
Humans
Intestinal Mucosa
Irritable Bowel Syndrome
Metagenome
Middle Aged
Polymerase Chain Reaction
Young Adult
Metadata
Show full item recordCitation
A molecular analysis of fecal and mucosal bacterial communities in irritable bowel syndrome. 2010, 55 (2):392-7 Dig. Dis. Sci.Journal
Digestive diseases and sciencesDOI
10.1007/s10620-009-0934-xPubMed ID
19693670Abstract
The objectives of this study were, firstly, to determine the diversity of the host's gut microbiota in irritable bowel syndrome (IBS) using a culture-independent method (DGGE of the 16S rRNA gene) and, secondly, to examine mucosal biopsies of IBS patients and compare them to their own fecal microbiota.The diversity of the dominant microbiota in the fecal material of IBS patients was compared to a healthy control group. In addition, we compared the mucosal and fecal microbiota of IBS patients.
Statistical analysis of the mean similarity data for these groups indicated a significant difference (P < 0.001) between IBS (n = 47) and healthy controls (n = 33) with significantly more variation in the gut microbiota of healthy volunteers than that of IBS patients. The average intra-individual similarity between the mucosa and luminal microbiota was 84%, which indicates that different communities were present at the two sites. This difference, however, is similar to that previously described between these two niches in control subjects. The average inter-individual similarity of the bacterial communities on the mucosa and in the lumen of IBS was not significantly different (P > 0.05).
IBS impacts equally on both bacterial communities in the IBS host and a significant difference in the gut microbiota exists between fecal samples from IBS patients and healthy controls. The reason for this difference is unclear and various possible explanations are available, but much more work is required to determine the underlying reason for this observation.
Item Type
ArticleLanguage
enISSN
1573-2568ae974a485f413a2113503eed53cd6c53
10.1007/s10620-009-0934-x
Scopus Count
Collections
Related articles
- Molecular analysis of the luminal- and mucosal-associated intestinal microbiota in diarrhea-predominant irritable bowel syndrome.
- Authors: Carroll IM, Ringel-Kulka T, Keku TO, Chang YH, Packey CD, Sartor RB, Ringel Y
- Issue date: 2011 Nov
- Identification of an Intestinal Microbiota Signature Associated With Severity of Irritable Bowel Syndrome.
- Authors: Tap J, Derrien M, Törnblom H, Brazeilles R, Cools-Portier S, Doré J, Störsrud S, Le Nevé B, Öhman L, Simrén M
- Issue date: 2017 Jan
- Increase in fecal primary bile acids and dysbiosis in patients with diarrhea-predominant irritable bowel syndrome.
- Authors: Duboc H, Rainteau D, Rajca S, Humbert L, Farabos D, Maubert M, Grondin V, Jouet P, Bouhassira D, Seksik P, Sokol H, Coffin B, Sabaté JM
- Issue date: 2012 Jun
- Fecal and Mucosa-Associated Intestinal Microbiota in Patients with Diarrhea-Predominant Irritable Bowel Syndrome.
- Authors: Maharshak N, Ringel Y, Katibian D, Lundqvist A, Sartor RB, Carroll IM, Ringel-Kulka T
- Issue date: 2018 Jul
- Ileocolonic Histopathological and Microbial Alterations in the Irritable Bowel Syndrome: A Nested Community Case-Control Study.
- Authors: Talley NJ, Alexander JL, Walker MM, Jones MP, Hugerth LW, Engstrand L, Agréus L, Powell N, Andreasson A
- Issue date: 2020 Dec 22