• Login
    View Item 
    •   Home
    • Hospital Research
    • Munster
    • Cork University Hospital
    • View Item
    •   Home
    • Hospital Research
    • Munster
    • Cork University Hospital
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Map of Submissions

    Home Page
    UlsterN
    4708
    UlsterS
    4708
    Connacht
    1603
    Munster
    48
    Leinster
    426

    Browse

    All of Lenus, The Irish Health RepositoryCommunitiesTitleAuthorsDate publishedSubjectsThis CollectionTitleAuthorsDate publishedSubjects

    My Account

    LoginRegister

    About

    About LenusDirectory of Open Access JournalsOpen Access Publishing GuideNational Health Library & Knowledge ServiceGuide to Publishers' PoliciesFAQsTerms and ConditionsVision StatementRIAN Pathways to Irish ResearchHSE position statement on Open AccessNational Open Research Forum (NORF)Zenodo (European Open Research repository)

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Food contaminant analysis at ultra-high mass resolution: application of hybrid linear ion trap - orbitrap mass spectrometry for the determination of the polyether toxins, azaspiracids, in shellfish.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Skrabáková, Zuzana
    O'Halloran, John
    van Pelt, Frank N A M
    James, Kevin J
    Affiliation
    PROTEOBIO (Mass Spectrometry Centre), Cork Institute of Technology, Bishopstown, Cork, Ireland.
    Issue Date
    2010-10-30
    MeSH
    Animals
    Food Contamination
    Marine Toxins
    Mytilus
    Reproducibility of Results
    Sensitivity and Specificity
    Shellfish
    Spiro Compounds
    Tandem Mass Spectrometry
    
    Metadata
    Show full item record
    Citation
    Food contaminant analysis at ultra-high mass resolution: application of hybrid linear ion trap - orbitrap mass spectrometry for the determination of the polyether toxins, azaspiracids, in shellfish. 2010, 24 (20):2966-74 Rapid Commun. Mass Spectrom.
    Publisher
    Wiley-Blackwell
    Journal
    Rapid communications in mass spectrometry : RCM
    URI
    http://hdl.handle.net/10147/200237
    DOI
    10.1002/rcm.4724
    PubMed ID
    20872629
    Abstract
    The biotoxins, azaspiracids (AZAs), from marine phytoplankton accumulate in shellfish and affect human health by causing severe gastrointestinal disturbance, diarrhea, nausea and vomiting. Specific and sensitive methods have been developed and validated for the determination of the most commonly occurring azaspiracid analogs. An LTQ Orbitrap mass spectrometer is a hybrid instrument that combines linear ion trap (LIT) mass spectrometry (MS) with high-resolution Fourier transform (FT) MS and this was exploited to perform simultaneous ultra-high-resolution full-scan MS analysis and collision-induced dissociation (CID) tandem mass spectrometry (MS/MS). Using the highest mass resolution setting (100,000 FWHM) in full-scan mode, the methodology was validated for the determination of six AZAs in mussel (Mytilus galloprovincialis) tissue extracts. Ultra-high mass resolution, together with a narrow mass tolerance window of ±2 mDa, dramatically improved detection sensitivity. In addition to employing chromatographic resolution to distinguish between the isomeric azaspiracid analogs, AZA1/AZA6 and AZA4/AZA5, higher energy collisionally induced dissociation (HCD) fragmentation on selected precursor ions were performed in parallel with full-scan FTMS. Using HCD MS/MS, most precursor and product ion masses were determined within 1 ppm of the theoretical m/z values throughout the mass spectral range and this enhanced the reliability of analyte identity.For the analysis of mussels (M. galloprovincialis), the method limit of quantitation (LOQ) was 0.010 µg/g using full-scan FTMS and this was comparable with the LOQ (0.007 µg/g) using CID MS/MS. The repeatability data were; intra-day RSD% (1.8-4.4%; n = 6) and inter-day RSD% (4.7-8.6%; n = 3). Application of these methods to the analysis of mussels (M. edulis) that were naturally contaminated with azaspiracids, using high-resolution full-scan Orbitrap MS and low-resolution CID MS/MS, produced equivalent quantitative data.
    Item Type
    Article
    Language
    en
    ISSN
    1097-0231
    ae974a485f413a2113503eed53cd6c53
    10.1002/rcm.4724
    Scopus Count
    Collections
    Cork University Hospital

    entitlement

    Related articles

    • Discovery of new analogs of the marine biotoxin azaspiracid in blue mussels (Mytilus edulis) by ultra-performance liquid chromatography/tandem mass spectrometry.
    • Authors: Rehmann N, Hess P, Quilliam MA
    • Issue date: 2008
    • Determination of azaspiracids in shellfish using liquid chromatography/tandem electrospray mass spectrometry.
    • Authors: Furey A, Braña-Magdalena A, Lehane M, Moroney C, James KJ, Satake M, Yasumoto T
    • Issue date: 2002
    • Performance of the EU-harmonised mouse bioassay for lipophilic toxins for the detection of azaspiracids in naturally contaminated mussel (Mytilus edulis) hepatopancreas tissue homogenates characterised by liquid chromatography coupled to tandem mass spectrometry.
    • Authors: Hess P, Butter T, Petersen A, Silke J, McMahon T
    • Issue date: 2009 Jun
    • Studies on azaspiracid biotoxins. II. Mass spectral behavior and structural elucidation of azaspiracid analogs.
    • Authors: Brombacher S, Edmonds S, Volmer DA
    • Issue date: 2002
    • Confirmation by LC-MS/MS of azaspiracids in shellfish from the Portuguese north-western coast.
    • Authors: Vale P, Bire R, Hess P
    • Issue date: 2008 Jun 15
    National Health Library & Knowledge Service | Health Service Executive | Dr Steevens' Hospital | Dublin 8 | Ireland
    lenus@hse.ie | Tel +353 (1) 6352558
    DSpace software copyright © 2002-2017  DuraSpace
    Contact Us | Disclaimer
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.