Composition, variability, and temporal stability of the intestinal microbiota of the elderly.
Authors
Claesson, Marcus JCusack, Siobhán
O'Sullivan, Orla
Greene-Diniz, Rachel
de Weerd, Heleen
Flannery, Edel
Marchesi, Julian R
Falush, Daniel
Dinan, Timothy
Fitzgerald, Gerald
Stanton, Catherine
van Sinderen, Douwe
O'Connor, Michael
Harnedy, Norma
O'Connor, Kieran
Henry, Colm
O'Mahony, Denis
Fitzgerald, Anthony P
Shanahan, Fergus
Twomey, Cillian
Hill, Colin
Ross, R Paul
O'Toole, Paul W
Affiliation
Department of Microbiology, University College, Cork, Ireland.Issue Date
2011-03-15MeSH
Age FactorsAged
Aged, 80 and over
Bacteria
Base Sequence
Cluster Analysis
Computational Biology
Feces
Humans
Intestines
Ireland
Metagenome
Molecular Sequence Data
Phylogeny
Principal Component Analysis
RNA, Ribosomal, 16S
Sequence Analysis, DNA
Species Specificity
Statistics, Nonparametric
Metadata
Show full item recordCitation
Composition, variability, and temporal stability of the intestinal microbiota of the elderly. 2011, 108 Suppl 1:4586-91 Proc. Natl. Acad. Sci. U.S.A.Journal
Proceedings of the National Academy of Sciences of the United States of AmericaDOI
10.1073/pnas.1000097107PubMed ID
20571116Additional Links
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3063589/pdf/pnas.201000097.pdfhttp://www.pnas.org/content/108/suppl.1/4586.full.pdf+html
Abstract
Alterations in the human intestinal microbiota are linked to conditions including inflammatory bowel disease, irritable bowel syndrome, and obesity. The microbiota also undergoes substantial changes at the extremes of life, in infants and older people, the ramifications of which are still being explored. We applied pyrosequencing of over 40,000 16S rRNA gene V4 region amplicons per subject to characterize the fecal microbiota in 161 subjects aged 65 y and older and 9 younger control subjects. The microbiota of each individual subject constituted a unique profile that was separable from all others. In 68% of the individuals, the microbiota was dominated by phylum Bacteroides, with an average proportion of 57% across all 161 baseline samples. Phylum Firmicutes had an average proportion of 40%. The proportions of some phyla and genera associated with disease or health also varied dramatically, including Proteobacteria, Actinobacteria, and Faecalibacteria. The core microbiota of elderly subjects was distinct from that previously established for younger adults, with a greater proportion of Bacteroides spp. and distinct abundance patterns of Clostridium groups. Analyses of 26 fecal microbiota datasets from 3-month follow-up samples indicated that in 85% of the subjects, the microbiota composition was more like the corresponding time-0 sample than any other dataset. We conclude that the fecal microbiota of the elderly shows temporal stability over limited time in the majority of subjects but is characterized by unusual phylum proportions and extreme variability.Item Type
ArticleLanguage
enISSN
1091-6490ae974a485f413a2113503eed53cd6c53
10.1073/pnas.1000097107
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