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    Validation of a norovirus multiplex real-time RT-PCR assay for the detection of norovirus GI and GII from faeces samples.

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    Authors
    Jones, S
    Douarre, P E
    O'Leary, J
    Corcoran, D
    O'Mahony, J
    Lucey, B
    Affiliation
    Department of Medical Microbiology, Cork University Hospital, Ireland.
    Issue Date
    2011
    MeSH
    Feces
    Humans
    Norovirus
    RNA, Viral
    Reverse Transcriptase Polymerase Chain Reaction
    
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    Citation
    Validation of a norovirus multiplex real-time RT-PCR assay for the detection of norovirus GI and GII from faeces samples. 2011, 68 (3):116-9 Br. J. Biomed. Sci.
    Journal
    British journal of biomedical science
    URI
    http://hdl.handle.net/10147/197879
    PubMed ID
    21950202
    Abstract
    Norovirus is a leading cause of infectious non-bacterial gastroenteritis. The virus is highly contagious and has multiple modes of transmission, presenting a growing challenge to hospital-based healthcare. In this study, a total of 120 stool samples are tested for the presence of norovirus GI and GII by the Roche two-step Lightcycler 2.0 assay incorporating primers and probes produced by TIB Molbiol, and the results are compared with results from the National Virus Reference Laboratory. The Roche/TIB Molbiol assay produced 51 positive results and 69 negative results. Discrepancy analysis was performed for six conflicting results using a second real-time polymerase chain reaction (PCR) assay (Roche/TIB Molbiol) and this confirmed that four of the five discrepant positive results were true positives. A single discrepant negative result generated by the Roche assay remained negative using the second assay. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were calculated to be 98%, 98.6%, 98.0% and 98.6%, respectively. Melting curve analysis was used to differentiate genogroups I and II and this showed that 92% of strains belonged to genogroup II.
    Item Type
    Article
    Language
    en
    ISSN
    0967-4845
    Collections
    Cork University Hospital

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