Folic acid modified gelatine coated quantum dots as potential reagents for in vitro cancer diagnostics
dc.contributor.author | Gerard, Valerie A | |
dc.contributor.author | Maguire, Ciaran M | |
dc.contributor.author | Bazou, Despina | |
dc.contributor.author | Gun'ko, Yurii K | |
dc.date.accessioned | 2011-12-07T09:44:38Z | |
dc.date.available | 2011-12-07T09:44:38Z | |
dc.date.issued | 2011-11-10 | |
dc.identifier | http://dx.doi.org/10.1186/1477-3155-9-50 | |
dc.identifier.citation | Journal of Nanobiotechnology. 2011 Nov 10;9(1):50 | |
dc.identifier.uri | http://hdl.handle.net/10147/196272 | |
dc.description.abstract | Abstract Background Gelatine coating was previously shown to effectively reduce the cytotoxicity of CdTe Quantum Dots (QDs) which was a first step towards utilising them for biomedical applications. To be useful they also need to be target-specific which can be achieved by conjugating them with Folic Acid (FA). Results The modification of QDs with FA via an original "one-pot" synthetic route was proved successful by a range of characterisation techniques including UV-visible absorption spectroscopy, Photoluminescence (PL) emission spectroscopy, fluorescence life-time measurements, Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS). The resulting nanocomposites were tested in Caco-2 cell cultures which over-express FA receptors. The presence of FA on the surface of QDs significantly improved the uptake by targeted cells. Conclusions The modification with folic acid enabled to achieve a significant cellular uptake and cytotoxicity towards a selected cancer cell lines (Caco-2) of gelatine-coated TGA-CdTe quantum dots, which demonstrated good potential for in vitro cancer diagnostics. | |
dc.title | Folic acid modified gelatine coated quantum dots as potential reagents for in vitro cancer diagnostics | |
dc.type | Journal Article | |
dc.language.rfc3066 | en | |
dc.rights.holder | Gerard et al.; licensee BioMed Central Ltd. | |
dc.description.status | Peer Reviewed | |
dc.date.updated | 2011-12-05T12:07:33Z | |
refterms.dateFOA | 2018-08-22T15:10:48Z | |
html.description.abstract | Abstract Background Gelatine coating was previously shown to effectively reduce the cytotoxicity of CdTe Quantum Dots (QDs) which was a first step towards utilising them for biomedical applications. To be useful they also need to be target-specific which can be achieved by conjugating them with Folic Acid (FA). Results The modification of QDs with FA via an original "one-pot" synthetic route was proved successful by a range of characterisation techniques including UV-visible absorption spectroscopy, Photoluminescence (PL) emission spectroscopy, fluorescence life-time measurements, Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS). The resulting nanocomposites were tested in Caco-2 cell cultures which over-express FA receptors. The presence of FA on the surface of QDs significantly improved the uptake by targeted cells. Conclusions The modification with folic acid enabled to achieve a significant cellular uptake and cytotoxicity towards a selected cancer cell lines (Caco-2) of gelatine-coated TGA-CdTe quantum dots, which demonstrated good potential for in vitro cancer diagnostics. |