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dc.contributor.authorGerard, Valerie A
dc.contributor.authorMaguire, Ciaran M
dc.contributor.authorBazou, Despina
dc.contributor.authorGun'ko, Yurii K
dc.date.accessioned2011-12-07T09:44:38Z
dc.date.available2011-12-07T09:44:38Z
dc.date.issued2011-11-10
dc.identifierhttp://dx.doi.org/10.1186/1477-3155-9-50
dc.identifier.citationJournal of Nanobiotechnology. 2011 Nov 10;9(1):50
dc.identifier.urihttp://hdl.handle.net/10147/196272
dc.description.abstractAbstract Background Gelatine coating was previously shown to effectively reduce the cytotoxicity of CdTe Quantum Dots (QDs) which was a first step towards utilising them for biomedical applications. To be useful they also need to be target-specific which can be achieved by conjugating them with Folic Acid (FA). Results The modification of QDs with FA via an original "one-pot" synthetic route was proved successful by a range of characterisation techniques including UV-visible absorption spectroscopy, Photoluminescence (PL) emission spectroscopy, fluorescence life-time measurements, Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS). The resulting nanocomposites were tested in Caco-2 cell cultures which over-express FA receptors. The presence of FA on the surface of QDs significantly improved the uptake by targeted cells. Conclusions The modification with folic acid enabled to achieve a significant cellular uptake and cytotoxicity towards a selected cancer cell lines (Caco-2) of gelatine-coated TGA-CdTe quantum dots, which demonstrated good potential for in vitro cancer diagnostics.
dc.titleFolic acid modified gelatine coated quantum dots as potential reagents for in vitro cancer diagnostics
dc.typeJournal Article
dc.language.rfc3066en
dc.rights.holderGerard et al.; licensee BioMed Central Ltd.
dc.description.statusPeer Reviewed
dc.date.updated2011-12-05T12:07:33Z
refterms.dateFOA2018-08-22T15:10:48Z
html.description.abstractAbstract Background Gelatine coating was previously shown to effectively reduce the cytotoxicity of CdTe Quantum Dots (QDs) which was a first step towards utilising them for biomedical applications. To be useful they also need to be target-specific which can be achieved by conjugating them with Folic Acid (FA). Results The modification of QDs with FA via an original "one-pot" synthetic route was proved successful by a range of characterisation techniques including UV-visible absorption spectroscopy, Photoluminescence (PL) emission spectroscopy, fluorescence life-time measurements, Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS). The resulting nanocomposites were tested in Caco-2 cell cultures which over-express FA receptors. The presence of FA on the surface of QDs significantly improved the uptake by targeted cells. Conclusions The modification with folic acid enabled to achieve a significant cellular uptake and cytotoxicity towards a selected cancer cell lines (Caco-2) of gelatine-coated TGA-CdTe quantum dots, which demonstrated good potential for in vitro cancer diagnostics.


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