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    Multiplex PCR to determine Streptococcus pneumoniae serotypes causing otitis media in the Republic of Ireland with further characterisation of antimicrobial susceptibilities and genotypes.

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    Authors
    Vickers, I
    O'Flanagan, D
    Cafferkey, M
    Humphreys, H
    Affiliation
    Epidemiology and Molecular Biology Unit and Irish Meningococcal and Meningitis Reference Laboratory, Children's University Hospital, Temple St., Dublin, Ireland. imelda.vickers@cuh.ie
    Issue Date
    2011-03
    MeSH
    Anti-Bacterial Agents
    Child
    Female
    Humans
    Ireland
    Male
    Microbial Sensitivity Tests
    Multilocus Sequence Typing
    Otitis Media
    Pneumococcal Infections
    Pneumococcal Vaccines
    Polymerase Chain Reaction
    Serotyping
    Streptococcus pneumoniae
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    Citation
    Multiplex PCR to determine Streptococcus pneumoniae serotypes causing otitis media in the Republic of Ireland with further characterisation of antimicrobial susceptibilities and genotypes. 2011, 30 (3):447-53 Eur. J. Clin. Microbiol. Infect. Dis.
    Journal
    European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
    URI
    http://hdl.handle.net/10147/144298
    DOI
    10.1007/s10096-010-1108-7
    PubMed ID
    21076929
    Additional Links
    http://www.springerlink.com/content/2tx3x15152025860/
    Abstract
    The purpose of this study was to determine the serotypes, genotypes and antimicrobial susceptibilities of Streptococcus pneumoniae causing otitis media (OM) in children in Dublin, Ireland. S. pneumoniae isolates (n = 28) from spontaneously discharging OM were studied. Serotyping was performed using a previously undescribed multiplex polymerase chain reaction (PCR) scheme in combination with serological methods. Multilocus sequence typing (MLST) was performed using standard procedures. Antimicrobial susceptibility testing was performed using the Etest method. Fourteen different S. pneumoniae serotypes were identified. The five most common serotypes were 3, 19F, 19A, 14 and 6A, which accounted for 68% of all infections. The 7-valent pneumococcal conjugate vaccine (PCV7), 10-valent pneumococcal conjugate vaccine (PHiD-CV) and 13-valent pneumococcal conjugate vaccine (PCV13) provided potential coverages of 43%, 46% and 86%, respectively. Reduced susceptibility to penicillin was evident for 25% of isolates and was associated with serotypes 14, 19A, 19F and 9V. A total of 21 different sequence types (STs) were identified. Pneumococcal Molecular Epidemiology Network (PMEN) clones or their variants represented 54% (15/28) of all isolates. Continued monitoring and characterisation of S. pneumoniae causing OM in Ireland is warranted in order to guide future vaccine and treatment policies.
    Item Type
    Article
    Language
    en
    ISSN
    1435-4373
    ae974a485f413a2113503eed53cd6c53
    10.1007/s10096-010-1108-7
    Scopus Count
    Collections
    Children's Health Ireland (CHI) at Temple St (formerly Children's University Hospital, Temple St)

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