Multiplex PCR to determine Streptococcus pneumoniae serotypes causing otitis media in the Republic of Ireland with further characterisation of antimicrobial susceptibilities and genotypes.
AffiliationEpidemiology and Molecular Biology Unit and Irish Meningococcal and Meningitis Reference Laboratory, Children's University Hospital, Temple St., Dublin, Ireland. email@example.com
Microbial Sensitivity Tests
Multilocus Sequence Typing
Polymerase Chain Reaction
MetadataShow full item record
CitationMultiplex PCR to determine Streptococcus pneumoniae serotypes causing otitis media in the Republic of Ireland with further characterisation of antimicrobial susceptibilities and genotypes. 2011, 30 (3):447-53 Eur. J. Clin. Microbiol. Infect. Dis.
JournalEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
AbstractThe purpose of this study was to determine the serotypes, genotypes and antimicrobial susceptibilities of Streptococcus pneumoniae causing otitis media (OM) in children in Dublin, Ireland. S. pneumoniae isolates (n = 28) from spontaneously discharging OM were studied. Serotyping was performed using a previously undescribed multiplex polymerase chain reaction (PCR) scheme in combination with serological methods. Multilocus sequence typing (MLST) was performed using standard procedures. Antimicrobial susceptibility testing was performed using the Etest method. Fourteen different S. pneumoniae serotypes were identified. The five most common serotypes were 3, 19F, 19A, 14 and 6A, which accounted for 68% of all infections. The 7-valent pneumococcal conjugate vaccine (PCV7), 10-valent pneumococcal conjugate vaccine (PHiD-CV) and 13-valent pneumococcal conjugate vaccine (PCV13) provided potential coverages of 43%, 46% and 86%, respectively. Reduced susceptibility to penicillin was evident for 25% of isolates and was associated with serotypes 14, 19A, 19F and 9V. A total of 21 different sequence types (STs) were identified. Pneumococcal Molecular Epidemiology Network (PMEN) clones or their variants represented 54% (15/28) of all isolates. Continued monitoring and characterisation of S. pneumoniae causing OM in Ireland is warranted in order to guide future vaccine and treatment policies.
- Etiology of Acute Otitis Media and Characterization of Pneumococcal Isolates After Introduction of 13-Valent Pneumococcal Conjugate Vaccine in Japanese Children.
- Authors: Ubukata K, Morozumi M, Sakuma M, Takata M, Mokuno E, Tajima T, Iwata S, AOM Surveillance Study Group
- Issue date: 2018 Jun
- Serotype Distribution, Antimicrobial Susceptibility, and Molecular Epidemiology of Streptococcus pneumoniae Isolated from Children in Shanghai, China.
- Authors: Pan F, Han L, Huang W, Tang J, Xiao S, Wang C, Qin H, Zhang H
- Issue date: 2015
- Epidemiological and molecular characterization of invasive Streptococcus pneumoniae isolated following introduction of 7-valent conjugate vaccine in Kinki region, Japan, 2008-2013.
- Authors: Toda H, Tanaka Y, Satoh K, Komatsu M, Wada Y, Tobe T, Kamisako T
- Issue date: 2020 May
- Serotype distribution and antibiotic resistance of Streptococcus pneumoniae isolates from 17 Chinese cities from 2011 to 2016.
- Authors: Zhao C, Li Z, Zhang F, Zhang X, Ji P, Zeng J, Hu B, Hu Z, Liao K, Sun H, Zhang R, Cao B, Zhuo C, Jia W, Mei Y, Chu Y, Xu X, Yang Q, Jin Y, Fu Q, Xu X, Li H, Wang L, Ni Y, Liang H, Wang H
- Issue date: 2017 Dec 29
- Serological and molecular capsular typing, antibiotic susceptibility and multilocus sequence typing of Streptococcus pneumoniae isolates from invasive and non-invasive infections.
- Authors: Zhang YJ, Chen YS, Wang ZW, Li YQ, Wang DX, Shang Y, Fu RR, Hu YH, Geng R, Wei LP, Yang JP, Li JS, Yu Q, DU J, Gao ZC
- Issue date: 2013 Jun