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    Amine functionalization of cholecyst-derived extracellular matrix with generation 1 PAMAM dendrimer.

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    Authors
    Chan, Jeffrey C Y
    Burugapalli, Krishna
    Naik, Hemantkumar
    Kelly, John L
    Pandit, Abhay
    Affiliation
    National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Republic of Ireland.
    Issue Date
    2008-02
    MeSH
    3T3 Cells
    Amines
    Animals
    Dendrimers
    Extracellular Matrix
    Gallbladder
    Mice
    Polyamines
    Swine
    
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    Citation
    Amine functionalization of cholecyst-derived extracellular matrix with generation 1 PAMAM dendrimer. 2008, 9 (2):528-36 Biomacromolecules
    Journal
    Biomacromolecules
    URI
    http://hdl.handle.net/10147/143797
    DOI
    10.1021/bm701055k
    PubMed ID
    18198835
    Additional Links
    http://dx.doi.org/10.1021/bm701055k
    Abstract
    A method to functionalize cholecyst-derived extracellular matrix (CEM) with free amine groups was established in an attempt to improve its potential for tethering of bioactive molecules. CEM was incorporated with Generation-1 polyamidoamine (G1 PAMAM) dendrimer by using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide and N-hydroxysuccinimide cross-linking system. The nature of incorporation of PAMAM dendrimer was evaluated using shrink temperature measurements, Fourier transform infrared (FTIR) assessment, ninhydrin assay, and swellability. The effects of PAMAM incorporation on mechanical and degradation properties of CEM were evaluated using a uniaxial mechanical test and collagenase degradation assay, respectively. Ninhydrin assay and FTIR assessment confirmed the presence of increasing free amine groups with increasing quantity of PAMAM in dendrimer-incorporated CEM (DENCEM) scaffolds. The amount of dendrimer used was found to be critical in controlling scaffold degradation, shrink temperature, and free amine content. Cell culture studies showed that fibroblasts seeded on DENCEM maintained their metabolic activity and ability to proliferate in vitro. In addition, fluorescence cell staining and scanning electron microscopy analysis of cell-seeded DENCEM showed preservation of normal fibroblast morphology and phenotype.
    Item Type
    Article
    Language
    en
    ISSN
    1526-4602
    ae974a485f413a2113503eed53cd6c53
    10.1021/bm701055k
    Scopus Count
    Collections
    Galway University Hospitals

    entitlement

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