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dc.contributor.authorBurugapalli, Krishna
dc.contributor.authorThapasimuttu, Anilkumar
dc.contributor.authorChan, Jeffrey C Y
dc.contributor.authorYao, Li
dc.contributor.authorBrody, Sarah
dc.contributor.authorKelly, Jack L
dc.contributor.authorPandit, Abhay
dc.date.accessioned2011-10-03T14:26:41Z
dc.date.available2011-10-03T14:26:41Z
dc.date.issued2007-03
dc.identifier.citationScaffold with a natural mesh-like architecture: isolation, structural, and in vitro characterization. 2007, 8 (3):928-36 Biomacromoleculesen
dc.identifier.issn1525-7797
dc.identifier.pmid17309297
dc.identifier.doi10.1021/bm061088x
dc.identifier.urihttp://hdl.handle.net/10147/143786
dc.descriptionAn intact extracellular matrix (ECM) with a mesh-like architecture has been identified in the peri-muscular sub-serosal connective tissue (PSCT) of cholecyst (gallbladder). The PSCT layer of cholecyst wall is isolated by mechanical delamination of other layers and decellularized with a treatment with peracetic acid and ethanol solution (PES) in water to obtain the final matrix, which is referred to as cholecyst-derived ECM (CEM). CEM is cross-linked with different concentrations of glutaraldehyde (GA) to demonstrate that the susceptibility of CEM to degradation can be controlled. Quantitative and qualitative macromolecular composition assessments revealed that collagen is the primary structural component of CEM. Elastin is also present. In addition, the ultra-structural studies on CEM reveal the presence of a three-dimensional fibrous mesh-like network structure with similar nanoscale architecture on both mucosal and serosal surfaces. In vitro cell culture studies show that CEM provides a supporting structure for the attachment and proliferation of murine fibroblasts (3T3) and human umbilical vein endothelial cells (HUVEC). CEM is also shown to support the attachment and differentiation of rat adrenal pheochromocytoma cells (PC12).en
dc.description.abstractAn intact extracellular matrix (ECM) with a mesh-like architecture has been identified in the peri-muscular sub-serosal connective tissue (PSCT) of cholecyst (gallbladder). The PSCT layer of cholecyst wall is isolated by mechanical delamination of other layers and decellularized with a treatment with peracetic acid and ethanol solution (PES) in water to obtain the final matrix, which is referred to as cholecyst-derived ECM (CEM). CEM is cross-linked with different concentrations of glutaraldehyde (GA) to demonstrate that the susceptibility of CEM to degradation can be controlled. Quantitative and qualitative macromolecular composition assessments revealed that collagen is the primary structural component of CEM. Elastin is also present. In addition, the ultra-structural studies on CEM reveal the presence of a three-dimensional fibrous mesh-like network structure with similar nanoscale architecture on both mucosal and serosal surfaces. In vitro cell culture studies show that CEM provides a supporting structure for the attachment and proliferation of murine fibroblasts (3T3) and human umbilical vein endothelial cells (HUVEC). CEM is also shown to support the attachment and differentiation of rat adrenal pheochromocytoma cells (PC12).
dc.language.isoenen
dc.relation.urlhttp://pubs.acs.org/doi/abs/10.1021/bm061088xen
dc.subject.meshAnimals
dc.subject.meshConnective Tissue
dc.subject.meshCross-Linking Reagents
dc.subject.meshElastin
dc.subject.meshEndothelium, Vascular
dc.subject.meshExtracellular Matrix
dc.subject.meshGallbladder
dc.subject.meshGlutaral
dc.subject.meshHumans
dc.subject.meshMice
dc.subject.meshMicroscopy, Electron, Scanning
dc.subject.meshNIH 3T3 Cells
dc.subject.meshPC12 Cells
dc.subject.meshRats
dc.subject.meshSwine
dc.titleScaffold with a natural mesh-like architecture: isolation, structural, and in vitro characterization.en
dc.typeArticleen
dc.contributor.departmentDepartment of Mechanical and Biomedical Engineering, National University of Ireland, Galway, Republic of Ireland.en
dc.identifier.journalBiomacromoleculesen
dc.description.provinceConnacht
html.description.abstractAn intact extracellular matrix (ECM) with a mesh-like architecture has been identified in the peri-muscular sub-serosal connective tissue (PSCT) of cholecyst (gallbladder). The PSCT layer of cholecyst wall is isolated by mechanical delamination of other layers and decellularized with a treatment with peracetic acid and ethanol solution (PES) in water to obtain the final matrix, which is referred to as cholecyst-derived ECM (CEM). CEM is cross-linked with different concentrations of glutaraldehyde (GA) to demonstrate that the susceptibility of CEM to degradation can be controlled. Quantitative and qualitative macromolecular composition assessments revealed that collagen is the primary structural component of CEM. Elastin is also present. In addition, the ultra-structural studies on CEM reveal the presence of a three-dimensional fibrous mesh-like network structure with similar nanoscale architecture on both mucosal and serosal surfaces. In vitro cell culture studies show that CEM provides a supporting structure for the attachment and proliferation of murine fibroblasts (3T3) and human umbilical vein endothelial cells (HUVEC). CEM is also shown to support the attachment and differentiation of rat adrenal pheochromocytoma cells (PC12).


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