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    Scaffold with a natural mesh-like architecture: isolation, structural, and in vitro characterization.

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    Authors
    Burugapalli, Krishna
    Thapasimuttu, Anilkumar
    Chan, Jeffrey C Y
    Yao, Li
    Brody, Sarah
    Kelly, Jack L
    Pandit, Abhay
    Affiliation
    Department of Mechanical and Biomedical Engineering, National University of Ireland, Galway, Republic of Ireland.
    Issue Date
    2007-03
    MeSH
    Animals
    Connective Tissue
    Cross-Linking Reagents
    Elastin
    Endothelium, Vascular
    Extracellular Matrix
    Gallbladder
    Glutaral
    Humans
    Mice
    Microscopy, Electron, Scanning
    NIH 3T3 Cells
    PC12 Cells
    Rats
    Swine
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    Citation
    Scaffold with a natural mesh-like architecture: isolation, structural, and in vitro characterization. 2007, 8 (3):928-36 Biomacromolecules
    Journal
    Biomacromolecules
    URI
    http://hdl.handle.net/10147/143786
    DOI
    10.1021/bm061088x
    PubMed ID
    17309297
    Additional Links
    http://pubs.acs.org/doi/abs/10.1021/bm061088x
    Abstract
    An intact extracellular matrix (ECM) with a mesh-like architecture has been identified in the peri-muscular sub-serosal connective tissue (PSCT) of cholecyst (gallbladder). The PSCT layer of cholecyst wall is isolated by mechanical delamination of other layers and decellularized with a treatment with peracetic acid and ethanol solution (PES) in water to obtain the final matrix, which is referred to as cholecyst-derived ECM (CEM). CEM is cross-linked with different concentrations of glutaraldehyde (GA) to demonstrate that the susceptibility of CEM to degradation can be controlled. Quantitative and qualitative macromolecular composition assessments revealed that collagen is the primary structural component of CEM. Elastin is also present. In addition, the ultra-structural studies on CEM reveal the presence of a three-dimensional fibrous mesh-like network structure with similar nanoscale architecture on both mucosal and serosal surfaces. In vitro cell culture studies show that CEM provides a supporting structure for the attachment and proliferation of murine fibroblasts (3T3) and human umbilical vein endothelial cells (HUVEC). CEM is also shown to support the attachment and differentiation of rat adrenal pheochromocytoma cells (PC12).
    Item Type
    Article
    Language
    en
    ISSN
    1525-7797
    ae974a485f413a2113503eed53cd6c53
    10.1021/bm061088x
    Scopus Count
    Collections
    Galway University Hospitals

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