Scaffold with a natural mesh-like architecture: isolation, structural, and in vitro characterization.
Authors
Burugapalli, KrishnaThapasimuttu, Anilkumar
Chan, Jeffrey C Y
Yao, Li
Brody, Sarah
Kelly, Jack L
Pandit, Abhay
Affiliation
Department of Mechanical and Biomedical Engineering, National University of Ireland, Galway, Republic of Ireland.Issue Date
2007-03MeSH
AnimalsConnective Tissue
Cross-Linking Reagents
Elastin
Endothelium, Vascular
Extracellular Matrix
Gallbladder
Glutaral
Humans
Mice
Microscopy, Electron, Scanning
NIH 3T3 Cells
PC12 Cells
Rats
Swine
Metadata
Show full item recordCitation
Scaffold with a natural mesh-like architecture: isolation, structural, and in vitro characterization. 2007, 8 (3):928-36 BiomacromoleculesJournal
BiomacromoleculesDOI
10.1021/bm061088xPubMed ID
17309297Additional Links
http://pubs.acs.org/doi/abs/10.1021/bm061088xAbstract
An intact extracellular matrix (ECM) with a mesh-like architecture has been identified in the peri-muscular sub-serosal connective tissue (PSCT) of cholecyst (gallbladder). The PSCT layer of cholecyst wall is isolated by mechanical delamination of other layers and decellularized with a treatment with peracetic acid and ethanol solution (PES) in water to obtain the final matrix, which is referred to as cholecyst-derived ECM (CEM). CEM is cross-linked with different concentrations of glutaraldehyde (GA) to demonstrate that the susceptibility of CEM to degradation can be controlled. Quantitative and qualitative macromolecular composition assessments revealed that collagen is the primary structural component of CEM. Elastin is also present. In addition, the ultra-structural studies on CEM reveal the presence of a three-dimensional fibrous mesh-like network structure with similar nanoscale architecture on both mucosal and serosal surfaces. In vitro cell culture studies show that CEM provides a supporting structure for the attachment and proliferation of murine fibroblasts (3T3) and human umbilical vein endothelial cells (HUVEC). CEM is also shown to support the attachment and differentiation of rat adrenal pheochromocytoma cells (PC12).Item Type
ArticleLanguage
enISSN
1525-7797ae974a485f413a2113503eed53cd6c53
10.1021/bm061088x