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dc.contributor.authorReeves, Emer P
dc.contributor.authorWilliamson, Michael
dc.contributor.authorO'Neill, Shane J
dc.contributor.authorGreally, Peter
dc.contributor.authorMcElvaney, Noel G
dc.date.accessioned2011-08-29T10:31:49Z
dc.date.available2011-08-29T10:31:49Z
dc.date.issued2011-06-01
dc.identifier.citationNebulized hypertonic saline decreases IL-8 in sputum of patients with cystic fibrosis. 2011, 183 (11):1517-23 Am. J. Respir. Crit. Care Med.en
dc.identifier.issn1535-4970
dc.identifier.pmid21330456
dc.identifier.doi10.1164/rccm.201101-0072OC
dc.identifier.urihttp://hdl.handle.net/10147/141079
dc.description.abstractInflammation within the cystic fibrosis (CF) lung is mediated by inflammatory chemokines, such as IL-8. IL-8 is protected from proteolytic degradation in the airways by binding to glycosaminoglycans, while remaining active. Evidence that increased hypertonicity of airway secretions induced by hypertonic saline treatment alters levels of IL-8 is lacking.
dc.description.abstractTo investigate the antiinflammatory effect of hypertonic saline (HTS) treatment within the CF lung by focusing on IL-8.
dc.description.abstractDegradation of IL-8 in CF lung secretions after treatment with glycosaminoglycan lyases and HTS was analyzed by Western blot analysis and ELISA. The ex vivo chemotactic activity of purified neutrophils in response to CF airway secretions was evaluated post nebulization of HTS (7% saline).
dc.description.abstractIn vivo CF bronchoalveolar lavage fluid (BALF) IL-8 levels were significantly higher than the control group (P < 0.05). Digesting glycosaminoglycans in CF BALF displaced IL-8 from glycosaminoglycan matrices, rendering the chemokine susceptible to proteolytic cleavage. High sodium concentrations also liberate IL-8 in CF BALF in vitro, and in vivo in CF sputum from patients receiving aerosolized HTS, resulting in degradation of IL-8 and decreased neutrophil chemotactic efficiency.
dc.description.abstractGlycosaminoglycans possess the ability to influence the chemokine profile of the CF lung by binding and stabilizing IL-8, which promotes neutrophil chemotaxis and activation. Nebulized hypertonic saline treatment disrupts the interaction between glycosaminoglycans and IL-8, rendering IL-8 susceptible to proteolytic degradation with subsequent decrease in neutrophil chemotaxis, thereby facilitating resolution of inflammation.
dc.language.isoenen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/21330456en
dc.subject.meshAdministration, Inhalation
dc.subject.meshAdult
dc.subject.meshBlotting, Western
dc.subject.meshBronchoalveolar Lavage Fluid
dc.subject.meshCystic Fibrosis
dc.subject.meshElectrophoresis, Polyacrylamide Gel
dc.subject.meshEnzyme-Linked Immunosorbent Assay
dc.subject.meshGlycosaminoglycans
dc.subject.meshHumans
dc.subject.meshInflammation
dc.subject.meshInterleukin-8
dc.subject.meshMiddle Aged
dc.subject.meshNebulizers and Vaporizers
dc.subject.meshSaline Solution, Hypertonic
dc.subject.meshSputum
dc.subject.meshYoung Adult
dc.titleNebulized hypertonic saline decreases IL-8 in sputum of patients with cystic fibrosis.en
dc.typeArticleen
dc.contributor.departmentRespiratory Research Division, Department of Medicine, Royal College of Surgeons in Ireland, Beaumont Hospital, Dublin 9, Ireland. emerreeves@rcsi.ieen
dc.identifier.journalAmerican journal of respiratory and critical care medicineen
dc.description.provinceLeinster
html.description.abstractInflammation within the cystic fibrosis (CF) lung is mediated by inflammatory chemokines, such as IL-8. IL-8 is protected from proteolytic degradation in the airways by binding to glycosaminoglycans, while remaining active. Evidence that increased hypertonicity of airway secretions induced by hypertonic saline treatment alters levels of IL-8 is lacking.
html.description.abstractTo investigate the antiinflammatory effect of hypertonic saline (HTS) treatment within the CF lung by focusing on IL-8.
html.description.abstractDegradation of IL-8 in CF lung secretions after treatment with glycosaminoglycan lyases and HTS was analyzed by Western blot analysis and ELISA. The ex vivo chemotactic activity of purified neutrophils in response to CF airway secretions was evaluated post nebulization of HTS (7% saline).
html.description.abstractIn vivo CF bronchoalveolar lavage fluid (BALF) IL-8 levels were significantly higher than the control group (P < 0.05). Digesting glycosaminoglycans in CF BALF displaced IL-8 from glycosaminoglycan matrices, rendering the chemokine susceptible to proteolytic cleavage. High sodium concentrations also liberate IL-8 in CF BALF in vitro, and in vivo in CF sputum from patients receiving aerosolized HTS, resulting in degradation of IL-8 and decreased neutrophil chemotactic efficiency.
html.description.abstractGlycosaminoglycans possess the ability to influence the chemokine profile of the CF lung by binding and stabilizing IL-8, which promotes neutrophil chemotaxis and activation. Nebulized hypertonic saline treatment disrupts the interaction between glycosaminoglycans and IL-8, rendering IL-8 susceptible to proteolytic degradation with subsequent decrease in neutrophil chemotaxis, thereby facilitating resolution of inflammation.


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