Nebulized hypertonic saline decreases IL-8 in sputum of patients with cystic fibrosis.
AffiliationRespiratory Research Division, Department of Medicine, Royal College of Surgeons in Ireland, Beaumont Hospital, Dublin 9, Ireland. email@example.com
Bronchoalveolar Lavage Fluid
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Nebulizers and Vaporizers
Saline Solution, Hypertonic
MetadataShow full item record
CitationNebulized hypertonic saline decreases IL-8 in sputum of patients with cystic fibrosis. 2011, 183 (11):1517-23 Am. J. Respir. Crit. Care Med.
JournalAmerican journal of respiratory and critical care medicine
AbstractInflammation within the cystic fibrosis (CF) lung is mediated by inflammatory chemokines, such as IL-8. IL-8 is protected from proteolytic degradation in the airways by binding to glycosaminoglycans, while remaining active. Evidence that increased hypertonicity of airway secretions induced by hypertonic saline treatment alters levels of IL-8 is lacking.
To investigate the antiinflammatory effect of hypertonic saline (HTS) treatment within the CF lung by focusing on IL-8.
Degradation of IL-8 in CF lung secretions after treatment with glycosaminoglycan lyases and HTS was analyzed by Western blot analysis and ELISA. The ex vivo chemotactic activity of purified neutrophils in response to CF airway secretions was evaluated post nebulization of HTS (7% saline).
In vivo CF bronchoalveolar lavage fluid (BALF) IL-8 levels were significantly higher than the control group (P < 0.05). Digesting glycosaminoglycans in CF BALF displaced IL-8 from glycosaminoglycan matrices, rendering the chemokine susceptible to proteolytic cleavage. High sodium concentrations also liberate IL-8 in CF BALF in vitro, and in vivo in CF sputum from patients receiving aerosolized HTS, resulting in degradation of IL-8 and decreased neutrophil chemotactic efficiency.
Glycosaminoglycans possess the ability to influence the chemokine profile of the CF lung by binding and stabilizing IL-8, which promotes neutrophil chemotaxis and activation. Nebulized hypertonic saline treatment disrupts the interaction between glycosaminoglycans and IL-8, rendering IL-8 susceptible to proteolytic degradation with subsequent decrease in neutrophil chemotaxis, thereby facilitating resolution of inflammation.
- LL-37 complexation with glycosaminoglycans in cystic fibrosis lungs inhibits antimicrobial activity, which can be restored by hypertonic saline.
- Authors: Bergsson G, Reeves EP, McNally P, Chotirmall SH, Greene CM, Greally P, Murphy P, O'Neill SJ, McElvaney NG
- Issue date: 2009 Jul 1
- The effect of the decoy molecule PA401 on CXCL8 levels in bronchoalveolar lavage fluid of patients with cystic fibrosis.
- Authors: McElvaney OJ, O'Reilly N, White M, Lacey N, Pohl K, Gerlza T, Bergin DA, Kerr H, McCarthy C, O'Brien ME, Adage T, Kungl AJ, Reeves EP, McElvaney NG
- Issue date: 2015 Feb
- The facilitating effect of hypertonic saline on resolution of airway inflammation in cystic fibrosis.
- Authors: Reeves EP, McElvaney NG
- Issue date: 2012 Jan 15
- Could you please pass the salt?
- Authors: Shoseyov D, Cohen-Cymberknoh M, Kerem E
- Issue date: 2011 Jun 1
- IL-8 dictates glycosaminoglycan binding and stability of IL-18 in cystic fibrosis.
- Authors: Reeves EP, Williamson M, Byrne B, Bergin DA, Smith SG, Greally P, O'Kennedy R, O'Neill SJ, McElvaney NG
- Issue date: 2010 Feb 1