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dc.contributor.authorRyan, James
dc.contributor.authorCurran, Catherine E
dc.contributor.authorHennessy, Emer
dc.contributor.authorNewell, John
dc.contributor.authorMorris, John C
dc.contributor.authorKerin, Michael J
dc.contributor.authorDwyer, Roisin M
dc.date.accessioned2011-08-29T10:09:01Z
dc.date.available2011-08-29T10:09:01Z
dc.date.issued2011-01
dc.identifier.citationThe sodium iodide symporter (NIS) and potential regulators in normal, benign and malignant human breast tissue. 2011, 6 (1):e16023 PLoS ONEen
dc.identifier.issn1932-6203
dc.identifier.pmid21283523
dc.identifier.doi10.1371/journal.pone.0016023
dc.identifier.urihttp://hdl.handle.net/10147/141047
dc.description.abstractThe presence, relevance and regulation of the Sodium Iodide Symporter (NIS) in human mammary tissue remains poorly understood. This study aimed to quantify relative expression of NIS and putative regulators in human breast tissue, with relationships observed further investigated in vitro.
dc.description.abstractHuman breast tissue specimens (malignant n = 75, normal n = 15, fibroadenoma n = 10) were analysed by RQ-PCR targeting NIS, receptors for retinoic acid (RARα, RARβ), oestrogen (ERα), thyroid hormones (THRα, THRβ), and also phosphoinositide-3-kinase (PI3K). Breast cancer cells were treated with Retinoic acid (ATRA), Estradiol and Thyroxine individually and in combination followed by analysis of changes in NIS expression.
dc.description.abstractThe lowest levels of NIS were detected in normal tissue (Mean(SEM) 0.70(0.12) Log(10) Relative Quantity (RQ)) with significantly higher levels observed in fibroadenoma (1.69(0.21) Log(10)RQ, p<0.005) and malignant breast tissue (1.18(0.07) Log(10)RQ, p<0.05). Significant positive correlations were observed between human NIS and ERα (r = 0.22, p<0.05) and RARα (r = 0.29, p<0.005), with the strongest relationship observed between NIS and RARβ (r = 0.38, p<0.0001). An inverse relationship between NIS and PI3K expression was also observed (r =  0.21, p<0.05). In vitro, ATRA, Estradiol and Thyroxine individually stimulated significant increases in NIS expression (range 6-16 fold), while ATRA and Thyroxine combined caused the greatest increase (range 16-26 fold).
dc.description.abstractAlthough NIS expression is significantly higher in malignant compared to normal breast tissue, the highest level was detected in fibroadenoma. The data presented supports a role for retinoic acid and estradiol in mammary NIS regulation in vivo, and also highlights potential thyroidal regulation of mammary NIS mediated by thyroid hormones.
dc.language.isoenen
dc.subject.meshBreast Neoplasms
dc.subject.meshEstradiol
dc.subject.meshFemale
dc.subject.meshFibroadenoma
dc.subject.meshGene Expression Regulation, Neoplastic
dc.subject.meshHumans
dc.subject.meshMammary Glands, Human
dc.subject.meshSymporters
dc.subject.meshThyroid Hormones
dc.subject.meshThyroxine
dc.subject.meshTretinoin
dc.titleThe sodium iodide symporter (NIS) and potential regulators in normal, benign and malignant human breast tissue.en
dc.typeArticleen
dc.contributor.departmentDivision of Surgery, School of Medicine, National University of Ireland Galway, Galway, Ireland.en
dc.identifier.journalPloS oneen
dc.description.provinceConnacht
refterms.dateFOA2018-08-22T13:28:40Z
html.description.abstractThe presence, relevance and regulation of the Sodium Iodide Symporter (NIS) in human mammary tissue remains poorly understood. This study aimed to quantify relative expression of NIS and putative regulators in human breast tissue, with relationships observed further investigated in vitro.
html.description.abstractHuman breast tissue specimens (malignant n = 75, normal n = 15, fibroadenoma n = 10) were analysed by RQ-PCR targeting NIS, receptors for retinoic acid (RARα, RARβ), oestrogen (ERα), thyroid hormones (THRα, THRβ), and also phosphoinositide-3-kinase (PI3K). Breast cancer cells were treated with Retinoic acid (ATRA), Estradiol and Thyroxine individually and in combination followed by analysis of changes in NIS expression.
html.description.abstractThe lowest levels of NIS were detected in normal tissue (Mean(SEM) 0.70(0.12) Log(10) Relative Quantity (RQ)) with significantly higher levels observed in fibroadenoma (1.69(0.21) Log(10)RQ, p<0.005) and malignant breast tissue (1.18(0.07) Log(10)RQ, p<0.05). Significant positive correlations were observed between human NIS and ERα (r = 0.22, p<0.05) and RARα (r = 0.29, p<0.005), with the strongest relationship observed between NIS and RARβ (r = 0.38, p<0.0001). An inverse relationship between NIS and PI3K expression was also observed (r =  0.21, p<0.05). In vitro, ATRA, Estradiol and Thyroxine individually stimulated significant increases in NIS expression (range 6-16 fold), while ATRA and Thyroxine combined caused the greatest increase (range 16-26 fold).
html.description.abstractAlthough NIS expression is significantly higher in malignant compared to normal breast tissue, the highest level was detected in fibroadenoma. The data presented supports a role for retinoic acid and estradiol in mammary NIS regulation in vivo, and also highlights potential thyroidal regulation of mammary NIS mediated by thyroid hormones.


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