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dc.contributor.authorGrogan, Juanita A
dc.contributor.authorLogan, Catriona
dc.contributor.authorO'Leary, John
dc.contributor.authorRush, Rebecca
dc.contributor.authorO'Sullivan, Niamh
dc.date.accessioned2011-07-27T11:25:24Z
dc.date.available2011-07-27T11:25:24Z
dc.date.issued2011-06
dc.identifier.citationReal-time PCR-based detection of Bordetella pertussis and Bordetella parapertussis in an Irish paediatric population. 2011, 60 (Pt 6):722-9 J. Med. Microbiol.en
dc.identifier.issn1473-5644
dc.identifier.pmid21393459
dc.identifier.doi10.1099/jmm.0.030049-0
dc.identifier.urihttp://hdl.handle.net/10147/137052
dc.description.abstractNovel real-time PCR assays targeting the Bordetella pertussis insertion sequence IS481, the toxin promoter region and Bordetella parapertussis insertion sequence IS1001 were designed. PCR assays were capable of detecting ≤10 copies of target DNA per reaction, with an amplification efficiency of ≥90 %. From September 2003 to December 2009, per-nasal swabs and nasopharyngeal aspirates submitted for B. pertussis culture from patients ≤1 month to >15 years of age were examined by real-time PCR. Among 1324 patients, 76 (5.7 %) were B. pertussis culture positive and 145 (10.95 %) were B. pertussis PCR positive. Of the B. pertussis PCR-positive patients, 117 (81 %) were aged 6 months or less. A total of 1548 samples were examined, of which 87 (5.6 %) were culture positive for B. pertussis and 169 (10.92 %) were B. pertussis PCR positive. All culture-positive samples were PCR positive. Seven specimens (0.5 %) were B. parapertussis culture positive and 10 (0.8 %) were B. parapertussis PCR positive, with all culture-positive samples yielding PCR-positive results. A review of patient laboratory records showed that of the 1324 patients tested for pertussis 555 (42 %) had samples referred for respiratory syncytial virus (RSV) testing and 165 (30 %) were positive, as compared to 19.4 % of the total 5719 patients tested for RSV in this period. Analysis of the age distribution of RSV-positive patients identified that 129 (78 %) were aged 6 months or less, similar to the incidence observed for pertussis in that patient age group. In conclusion, the introduction of the real-time PCR assays for the routine detection of B. pertussis resulted in a 91 % increase in the detection of the organism as compared to microbiological culture. The incidence of infection with B. parapertussis is low while the incidence of RSV infection in infants suspected of having pertussis is high, with a similar age distribution to B. pertussis infection.
dc.language.isoenen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/21393459en
dc.subject.meshAdolescent
dc.subject.meshBacteriological Techniques
dc.subject.meshBordetella Infections
dc.subject.meshBordetella parapertussis
dc.subject.meshBordetella pertussis
dc.subject.meshChild
dc.subject.meshChild, Preschool
dc.subject.meshHumans
dc.subject.meshIncidence
dc.subject.meshInfant
dc.subject.meshInfant, Newborn
dc.subject.meshIreland
dc.subject.meshNasal Mucosa
dc.subject.meshNasopharynx
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshSensitivity and Specificity
dc.titleReal-time PCR-based detection of Bordetella pertussis and Bordetella parapertussis in an Irish paediatric population.en
dc.typeArticleen
dc.contributor.departmentDepartment of Microbiology, Our Lady's Children's Hospital, Dublin, Ireland. juanita.grogan@olchc.ieen
dc.identifier.journalJournal of medical microbiologyen
dc.description.provinceLeinster
html.description.abstractNovel real-time PCR assays targeting the Bordetella pertussis insertion sequence IS481, the toxin promoter region and Bordetella parapertussis insertion sequence IS1001 were designed. PCR assays were capable of detecting ≤10 copies of target DNA per reaction, with an amplification efficiency of ≥90 %. From September 2003 to December 2009, per-nasal swabs and nasopharyngeal aspirates submitted for B. pertussis culture from patients ≤1 month to >15 years of age were examined by real-time PCR. Among 1324 patients, 76 (5.7 %) were B. pertussis culture positive and 145 (10.95 %) were B. pertussis PCR positive. Of the B. pertussis PCR-positive patients, 117 (81 %) were aged 6 months or less. A total of 1548 samples were examined, of which 87 (5.6 %) were culture positive for B. pertussis and 169 (10.92 %) were B. pertussis PCR positive. All culture-positive samples were PCR positive. Seven specimens (0.5 %) were B. parapertussis culture positive and 10 (0.8 %) were B. parapertussis PCR positive, with all culture-positive samples yielding PCR-positive results. A review of patient laboratory records showed that of the 1324 patients tested for pertussis 555 (42 %) had samples referred for respiratory syncytial virus (RSV) testing and 165 (30 %) were positive, as compared to 19.4 % of the total 5719 patients tested for RSV in this period. Analysis of the age distribution of RSV-positive patients identified that 129 (78 %) were aged 6 months or less, similar to the incidence observed for pertussis in that patient age group. In conclusion, the introduction of the real-time PCR assays for the routine detection of B. pertussis resulted in a 91 % increase in the detection of the organism as compared to microbiological culture. The incidence of infection with B. parapertussis is low while the incidence of RSV infection in infants suspected of having pertussis is high, with a similar age distribution to B. pertussis infection.


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