Bioluminescent imaging: a critical tool in pre-clinical oncology research.
Affiliation
UCD School of Medicine and Medical Science, Health Science Building, University College Dublin, Belfield, Dublin 4, Ireland.Issue Date
2010-02MeSH
AnimalsDisease Models, Animal
Disease Progression
Luciferases
Luminescent Measurements
Mice
Neoplasm Metastasis
Neoplasms, Experimental
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Bioluminescent imaging: a critical tool in pre-clinical oncology research. 2010, 220 (3):317-27 J. Pathol.Journal
The Journal of pathologyDOI
10.1002/path.2656PubMed ID
19967724Abstract
Bioluminescent imaging (BLI) is a non-invasive imaging modality widely used in the field of pre-clinical oncology research. Imaging of small animal tumour models using BLI involves the generation of light by luciferase-expressing cells in the animal following administration of substrate. This light may be imaged using an external detector. The technique allows a variety of tumour-associated properties to be visualized dynamically in living models. The increasing use of BLI as a small-animal imaging modality has led to advances in the development of xenogeneic, orthotopic, and genetically engineered animal models expressing luciferase genes. This review aims to provide insight into the principles of BLI and its applications in cancer research. Many studies to assess tumour growth and development, as well as efficacy of candidate therapeutics, have been performed using BLI. More recently, advances have also been made using bioluminescent imaging in studies of protein-protein interactions, genetic screening, cell-cycle regulators, and spontaneous cancer development. Such novel studies highlight the versatility and potential of bioluminescent imaging in future oncological research.Item Type
ArticleLanguage
enISSN
1096-9896ae974a485f413a2113503eed53cd6c53
10.1002/path.2656
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