The Vibrio parahaemolyticus Type III Secretion Systems manipulate host cell MAPK for critical steps in pathogenesis.
O'Byrne, Conor P
Baird, Alan W
Coffey, Eleanor T
AffiliationDiscipline of Microbiology, School of Natural Sciences, National University of Ireland, Galway, University Road, Galway, Ireland.
Membrane Transport Proteins
Mitogen-Activated Protein Kinases
MetadataShow full item record
CitationThe Vibrio parahaemolyticus Type III Secretion Systems manipulate host cell MAPK for critical steps in pathogenesis. 2010, 10:329 BMC Microbiol.
AbstractVibrio parahaemolyticus is a food-borne pathogen causing inflammation of the gastrointestinal epithelium. Pathogenic strains of this bacterium possess two Type III Secretion Systems (TTSS) that deliver effector proteins into host cells. In order to better understand human host cell responses to V. parahaemolyticus, the modulation of Mitogen Activated Protein Kinase (MAPK) activation in epithelial cells by an O3:K6 clinical isolate, RIMD2210633, was investigated. The importance of MAPK activation for the ability of the bacterium to be cytotoxic and to induce secretion of Interleukin-8 (IL-8) was determined.
V. parahaemolyticus deployed its TTSS1 to induce activation of the JNK, p38 and ERK MAPK in human epithelial cells. VP1680 was identified as the TTSS1 effector protein responsible for MAPK activation in Caco-2 cells and the activation of JNK and ERK by this protein was important in induction of host cell death. V. parahaemolyticus actively induced IL-8 secretion in a response mediated by TTSS1. A role for VP1680 and for the ERK signalling pathway in the stimulation of IL-8 production in epithelial cells by V. parahaemolyticus was established. Interestingly, TTSS2 inhibited IL-8 mRNA transcription at early stages of interaction between the bacterium and the cell.
This study demonstrated that V. parahaemolyticus activates the three major MAPK signalling pathways in intestinal epithelial cells in a TTSS1-dependent manner that involves the TTSS1 effector VP1680. Furthermore VP1680 and JNK and ERK activation were needed for maximal cytotoxicity of the bacterium. It was shown that V. parahaemolyticus is a strong inducer of IL-8 secretion and that induction reflects a balance between the effects of TTSS1 and TTSS2. Increases in IL-8 secretion were mediated by TTSS1 and VP1680, and augmented by ERK activation. These results shed light on the mechanisms of bacterial pathogenesis mediated by TTSS and suggest significant roles for MAPK signalling during infection with V. parahaemolyticus.
- Vibrio parahaemolyticus infection induces modulation of IL-8 secretion through dual pathway via VP1680 in Caco-2 cells.
- Authors: Shimohata T, Nakano M, Lian X, Shigeyama T, Iba H, Hamamoto A, Yoshida M, Harada N, Yamamoto H, Yamato M, Mawatari K, Tamaki T, Nakaya Y, Takahashi A
- Issue date: 2011 Feb 15
- Manipulation of intestinal epithelial cell function by the cell contact-dependent type III secretion systems of Vibrio parahaemolyticus.
- Authors: O'Boyle N, Boyd A
- Issue date: 2014
- Identification of proteins secreted via Vibrio parahaemolyticus type III secretion system 1.
- Authors: Ono T, Park KS, Ueta M, Iida T, Honda T
- Issue date: 2006 Feb
- Functional characterization of two type III secretion systems of Vibrio parahaemolyticus.
- Authors: Park KS, Ono T, Rokuda M, Jang MH, Okada K, Iida T, Honda T
- Issue date: 2004 Nov
- Cell death mediated by Vibrio parahaemolyticus type III secretion system 1 is dependent on ERK1/2 MAPK, but independent of caspases.
- Authors: Yang YJ, Lee NK, Lee NY, Lee JW, Park SJ
- Issue date: 2011 Sep