Transcriptional regulation of human FE65, a ligand of Alzheimer's disease amyloid precursor protein, by Sp1.
Authors
Yu, Hoi-TinChan, William Wai-Lun
Chai, Ka-Ho
Lee, Chris Wing-Cheung
Chang, Raymond Chuen-Chung
Yu, Man-Shan
McLoughlin, Declan M
Miller, Christopher C J
Lau, Kwok-Fai
Affiliation
Department of Biochemistry (Science), The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR.Issue Date
2010-03-01MeSH
Amyloid beta-Protein PrecursorGene Expression Regulation
Humans
Nerve Tissue Proteins
Neurons
Nuclear Proteins
Promoter Regions, Genetic
RNA, Messenger
Sp1 Transcription Factor
Transcription, Genetic
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Transcriptional regulation of human FE65, a ligand of Alzheimer's disease amyloid precursor protein, by Sp1. 2010, 109 (4):782-93 J. Cell. Biochem.Journal
Journal of cellular biochemistryDOI
10.1002/jcb.22457PubMed ID
20091743Additional Links
http://dx.doi.org/10.1002/jcb.22457Abstract
FE65 is a neuronal-enriched adaptor protein that binds to the Alzheimer's disease amyloid precursor protein (APP). FE65 forms a transcriptionally active complex with the APP intracellular domain (AICD). The precise gene targets for this complex are unclear but several Alzheimer's disease-linked genes have been proposed. Additionally, evidence suggests that FE65 influences APP metabolism. The mechanism by which FE65 expression is regulated is as yet unknown. To gain insight into the regulatory mechanism, we cloned a 1.6 kb fragment upstream of the human FE65 gene and found that it possesses particularly strong promoter activity in neurones. To delineate essential regions in the human FE65 promoter, a series of deletion mutants were generated. The minimal FE65 promoter was located between -100 and +5, which contains a functional Sp1 site. Overexpression of the transcription factor Sp1 potentiates the FE65 promoter activity. Conversely, suppression of the FE65 promoter was observed in cells either treated with an Sp1 inhibitor or in which Sp1 was knocked down. Furthermore, reduced levels of Sp1 resulted in downregulation of endogenous FE65 mRNA and protein. These findings reveal that Sp1 plays a crucial role in transcriptional control of the human FE65 gene.Language
enISSN
1097-4644ae974a485f413a2113503eed53cd6c53
10.1002/jcb.22457
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