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    Biofilm characteristics of Staphylococcus epidermidis isolates associated with device-related meningitis.

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    Authors
    Stevens, Niall T
    Greene, Catherine M
    O'Gara, James P
    Humphreys, Hilary
    Affiliation
    Department of Clinical Microbiology, Royal College of Surgeons in Ireland Education & Research Centre, Beaumont Hospital, Dublin, Ireland.
    Issue Date
    2009-07
    MeSH
    Bacterial Proteins
    Biofilms
    Equipment and Supplies
    Gene Expression Regulation, Bacterial
    Humans
    Meningitis, Bacterial
    Staphylococcal Infections
    Staphylococcus epidermidis
    
    Metadata
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    Citation
    Biofilm characteristics of Staphylococcus epidermidis isolates associated with device-related meningitis. 2009, 58 (Pt 7):855-62 J. Med. Microbiol.
    Journal
    Journal of medical microbiology
    URI
    http://hdl.handle.net/10147/127505
    DOI
    10.1099/jmm.0.009209-0
    PubMed ID
    19502363
    Additional Links
    http://jmm.sgmjournals.org/cgi/reprint/58/7/855
    Abstract
    Staphylococcus epidermidis biofilm causes device-related meningitis in neurosurgical patients. This study assessed the contribution of polysaccharide and protein to the development of a strong biofilm-positive phenotype in four S. epidermidis isolates associated with probable device-related meningitis, under varying environmental conditions. RT-PCR analysis of the intercellular adhesion operon (icaADBC) and assessment of polysaccharide intercellular adhesin (PIA) production indicated a correlation between increased icaA transcription and PIA production in ica(+) isolates grown in medium with 4 % ethanol and 4 % NaCl. Treatment of biofilm with sodium metaperiodate caused dispersion of adhered cells (P <0.0001), indicating involvement of PIA. Transcriptional levels of protein factors revealed that atlE transcription levels were similar in all isolates, whilst aap levels were variable, with induction being seen in two isolates following growth in the presence of alcohol or salt. Transcription of agr did not influence protein expression and RNAIII transcription varied among the strains. Although aap transcription was induced, the treatment of biofilm with proteinase K did not always disperse the biofilm. Our data suggest that, among the three ica(+) S. epidermidis isolates clinically associated with meningitis that were studied, PIA contributed to the strong biofilm-positive phenotype, whereas protein factors appeared to have a secondary role.
    Item Type
    Article
    Language
    en
    ISSN
    0022-2615
    ae974a485f413a2113503eed53cd6c53
    10.1099/jmm.0.009209-0
    Scopus Count
    Collections
    Beaumont Hospital

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