Biofilm characteristics of Staphylococcus epidermidis isolates associated with device-related meningitis.
Affiliation
Department of Clinical Microbiology, Royal College of Surgeons in Ireland Education & Research Centre, Beaumont Hospital, Dublin, Ireland.Issue Date
2009-07MeSH
Bacterial ProteinsBiofilms
Equipment and Supplies
Gene Expression Regulation, Bacterial
Humans
Meningitis, Bacterial
Staphylococcal Infections
Staphylococcus epidermidis
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Biofilm characteristics of Staphylococcus epidermidis isolates associated with device-related meningitis. 2009, 58 (Pt 7):855-62 J. Med. Microbiol.Journal
Journal of medical microbiologyDOI
10.1099/jmm.0.009209-0PubMed ID
19502363Additional Links
http://jmm.sgmjournals.org/cgi/reprint/58/7/855Abstract
Staphylococcus epidermidis biofilm causes device-related meningitis in neurosurgical patients. This study assessed the contribution of polysaccharide and protein to the development of a strong biofilm-positive phenotype in four S. epidermidis isolates associated with probable device-related meningitis, under varying environmental conditions. RT-PCR analysis of the intercellular adhesion operon (icaADBC) and assessment of polysaccharide intercellular adhesin (PIA) production indicated a correlation between increased icaA transcription and PIA production in ica(+) isolates grown in medium with 4 % ethanol and 4 % NaCl. Treatment of biofilm with sodium metaperiodate caused dispersion of adhered cells (P <0.0001), indicating involvement of PIA. Transcriptional levels of protein factors revealed that atlE transcription levels were similar in all isolates, whilst aap levels were variable, with induction being seen in two isolates following growth in the presence of alcohol or salt. Transcription of agr did not influence protein expression and RNAIII transcription varied among the strains. Although aap transcription was induced, the treatment of biofilm with proteinase K did not always disperse the biofilm. Our data suggest that, among the three ica(+) S. epidermidis isolates clinically associated with meningitis that were studied, PIA contributed to the strong biofilm-positive phenotype, whereas protein factors appeared to have a secondary role.Item Type
ArticleLanguage
enISSN
0022-2615ae974a485f413a2113503eed53cd6c53
10.1099/jmm.0.009209-0
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