Taurine modulates neutrophil function but potentiates uropathogenic E. coli infection in the murine bladder.
Authors
Condron, ClaireCasey, Rowan G
Kehoe, Siobhan
Toomey, Deirdre
Creagh, Tom
Bouchier-Hayes, David J
Affiliation
Department of Surgery, Royal College of Surgeons in Ireland, Beaumont Hospital, Dublin, Ireland.Issue Date
2010-08MeSH
AnimalsDisease Models, Animal
Escherichia coli
Escherichia coli Infections
Female
Mice
Mice, Inbred C57BL
Neutrophil Infiltration
Neutrophils
Taurine
Urinary Bladder
Urinary Tract Infections
Urothelium
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Taurine modulates neutrophil function but potentiates uropathogenic E. coli infection in the murine bladder. 2010, 38 (4):215-22 Urol. Res.Journal
Urological researchDOI
10.1007/s00240-009-0235-zPubMed ID
19940987Abstract
Eradication of a urinary tract infection (UTI) appears to be related to a number of innate host defence mechanisms and their interactions with invading bacteria. Recurrent UTIs (rUTIs) pose a difficult problem in that these bacteria use both host and bacterial factors to evade elimination. Neutrophil bactericidal function is depressed, both systemically and in urine, in patients with a history of recurrent UTI. Taurine is a semi-essential amino acid and is successful in preserving neutrophil bactericidal function in urine. Taurine may preserve neutrophil function at the urothelium and thus aid UTI resolution. Adult female (6 weeks old) C57Bl/6 mice were randomised into three groups: a saline gavage only control group, a saline gavage + E. coli group, and a taurine gavage + E. coli group [21 g/70 kg taurine in 0.9% normal saline (N/S) for 5 days]. Whilst taurine gavage pre-treatment resulted in increased serum neutrophils respiratory burst activity, at the urothelial-endothelial interface it caused higher colony forming units in the urine and a higher incidence of E. coli invasion in the bladder wall with no evidence of increased bladder wall neutrophils infiltration on MPO assay of histological assessment. Histologically there was also evidence of reduced bladder inflammation and urothelial cell apoptosis. In conclusion, taurine effectively increases neutrophils activity but given its anti-inflammatory properties, at the expense of decreased urothelial-endothelial activation thus preventing clearance of active E. coli infection in the bladder. Despite the negative results, this study demonstrates the importance of modulating interactions at the urothelial interface.Item Type
ArticleLanguage
enISSN
1434-0879ae974a485f413a2113503eed53cd6c53
10.1007/s00240-009-0235-z
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