Iodinated contrast media alter immune responses in pro-inflammatory states.
AffiliationDepartment of Radiology and Vascular Surgery, Beaumont Hospital and Royal College of Surgeons in Ireland, Dublin, Ireland.
MetadataShow full item record
CitationIodinated contrast media alter immune responses in pro-inflammatory states. 2010, 51 (6):635-40 Acta Radiol
JournalActa radiologica (Stockholm, Sweden : 1987)
AbstractHypertonic saline causes a transient elevation of blood osmolality and has been shown to alter cellular inflammatory responses in pro-inflammatory states. Intravascular administration of iodine contrast media also causes a transient elevation of blood osmolarity.
To investigate the effect of iodine contrast media on leukocyte-endothelial interaction in vivo using intravital microscopy.
Male Sprague-Dawley rats (n=36) were randomized into six groups and were treated with: saline, high osmolar contrast medium, low osmolar contrast medium, and endotoxin alone and in combination with the high and low osmolar contrast media. The effect on leukocyte-endothelial interaction in the post-mesenteric venules was observed using the technique of intravital microscopy. The sequence of leukocyte rolling velocity, leukocyte-endothelial cell adherence, and transmigration was recorded and analyzed at 10 min intervals to a maximum of 120 min.
Endotoxin significantly decreased the rolling velocity and increased the adherence and transmigration of leukocytes in a time-dependent manner. Both types of iodine contrast media attenuated this pro-inflammatory response to endotoxin. This effect began between 60 and 70 min after the onset of the experiment.
Hyperosmolar and lower osmolar iodine contrast media attenuate the pro-inflammatory leukocyte-endothelial response to endotoxin in vivo.