Iodinated contrast media alter immune responses in pro-inflammatory states.
Affiliation
Department of Radiology and Vascular Surgery, Beaumont Hospital and Royal College of Surgeons in Ireland, Dublin, Ireland.Issue Date
2010-07MeSH
AnimalsCell Adhesion
Contrast Media
Endothelial Cells
Endotoxins
Halogenation
Immunity, Cellular
Inflammation
Iodine
Leukocytes
Male
Osmolar Concentration
Rats
Rats, Sprague-Dawley
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Iodinated contrast media alter immune responses in pro-inflammatory states. 2010, 51 (6):635-40 Acta RadiolJournal
Acta radiologica (Stockholm, Sweden : 1987)DOI
10.3109/02841851.2010.481679PubMed ID
20528648Abstract
Hypertonic saline causes a transient elevation of blood osmolality and has been shown to alter cellular inflammatory responses in pro-inflammatory states. Intravascular administration of iodine contrast media also causes a transient elevation of blood osmolarity.To investigate the effect of iodine contrast media on leukocyte-endothelial interaction in vivo using intravital microscopy.
Male Sprague-Dawley rats (n=36) were randomized into six groups and were treated with: saline, high osmolar contrast medium, low osmolar contrast medium, and endotoxin alone and in combination with the high and low osmolar contrast media. The effect on leukocyte-endothelial interaction in the post-mesenteric venules was observed using the technique of intravital microscopy. The sequence of leukocyte rolling velocity, leukocyte-endothelial cell adherence, and transmigration was recorded and analyzed at 10 min intervals to a maximum of 120 min.
Endotoxin significantly decreased the rolling velocity and increased the adherence and transmigration of leukocytes in a time-dependent manner. Both types of iodine contrast media attenuated this pro-inflammatory response to endotoxin. This effect began between 60 and 70 min after the onset of the experiment.
Hyperosmolar and lower osmolar iodine contrast media attenuate the pro-inflammatory leukocyte-endothelial response to endotoxin in vivo.
Item Type
ArticleLanguage
enISSN
1600-0455ae974a485f413a2113503eed53cd6c53
10.3109/02841851.2010.481679
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