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    Measurement of the unfolded protein response (UPR) in monocytes.

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    Authors
    Carroll, Tomás P
    Greene, Catherine M
    McElvaney, Noel G
    Affiliation
    Department of Medicine, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin, Ireland.
    Issue Date
    2011
    
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    Citation
    Measurement of the unfolded protein response (UPR) in monocytes. 2011, 489:83-95 Meth. Enzymol.
    Journal
    Methods in enzymology
    URI
    http://hdl.handle.net/10147/126092
    DOI
    10.1016/B978-0-12-385116-1.00005-4
    PubMed ID
    21266225
    Abstract
    In mammalian cells, the primary function of the endoplasmic reticulum (ER) is to synthesize and assemble membrane and secreted proteins. As the main site of protein folding and posttranslational modification in the cell, the ER operates a highly conserved quality control system to ensure only correctly assembled proteins exit the ER and misfolded and unfolded proteins are retained for disposal. Any disruption in the equilibrium of the ER engages a multifaceted intracellular signaling pathway termed the unfolded protein response (UPR) to restore normal conditions in the cell. A variety of pathological conditions can induce activation of the UPR, including neurodegenerative disorders such as Parkinson's disease, metabolic disorders such as atherosclerosis, and conformational disorders such as cystic fibrosis. Conformational disorders are characterized by mutations that modify the final structure of a protein and any cells that express abnormal protein risk functional impairment. The monocyte is an important and long-lived immune cell and acts as a key immunological orchestrator, dictating the intensity and duration of the host immune response. Monocytes expressing misfolded or unfolded protein may exhibit UPR activation and this can compromise the host immune system. Here, we describe in detail methods and protocols for the examination of UPR activation in peripheral blood monocytes. This guide should provide new investigators to the field with a broad understanding of the tools required to investigate the UPR in the monocyte.
    Item Type
    Article
    Language
    en
    ISSN
    1557-7988
    ae974a485f413a2113503eed53cd6c53
    10.1016/B978-0-12-385116-1.00005-4
    Scopus Count
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    Beaumont Hospital

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