Characterization of a Novel Arginine Catabolic Mobile Element (ACME) and Staphylococcal Chromosomal Cassette mec Composite Island with Significant Homology to Staphylococcus epidermidis ACME type II in Methicillin-Resistant Staphylococcus aureus Genotype ST22-MRSA-IV.
Authors
Shore, Anna CRossney, Angela S
Brennan, Orla M
Kinnevey, Peter M
Humphreys, Hilary
Sullivan, Derek J
Goering, Richard V
Ehricht, Ralf
Monecke, Stefan
Coleman, David C
Affiliation
Microbiology Research Unit, Dublin Dental University Hospital, University of Dublin, Trinity College Dublin, Ireland; National MRSA Reference Laboratory, Dublin, Ireland; Department of Clinical Microbiology, The Royal College of Surgeons in Ireland, Dublin, Ireland; Department of Microbiology, Beaumont Hospital, Dublin, Ireland; Creighton University, Omaha, Nebraska, USA; Alere Technologies GmbH, Jena, Germany; Institute for Medical Microbiology and Hygiene, Faculty of Medicine "Carl Gustav Carus", Technical University of Dresden, Germany.Issue Date
2011-02-22
Metadata
Show full item recordCitation
Characterization of a Novel Arginine Catabolic Mobile Element (ACME) and Staphylococcal Chromosomal Cassette mec Composite Island with Significant Homology to Staphylococcus epidermidis ACME type II in Methicillin-Resistant Staphylococcus aureus Genotype ST22-MRSA-IV. 2011:notAntimicrob Agents ChemotherJournal
Antimicrobial agents and chemotherapyDOI
10.1128/AAC.01756-10PubMed ID
21343442Abstract
The arginine catabolic mobile element (ACME) is prevalent among ST8-MRSA-IVa (USA300) isolates and evidence suggests that ACME enhances the ability of ST8-MRSA-IVa to grow and survive on its host. ACME has been identified in a small number of isolates belonging to other MRSA clones but is widespread among coagulase-negative staphylococci (CoNS). This study reports the first description of ACME in two distinct strains of the pandemic ST22-MRSA-IV clone. A total of 238 MRSA isolates recovered in Ireland between 1971 and 2008 were investigated for ACME using a DNA microarray. Twenty-three isolates (9.7%) were ACME-positive, all were either MRSA genotype ST8-MRSA-IVa (7/23, 30%) or ST22-MRSA-IV (16/23, 70%). Whole-genome sequencing and comprehensive molecular characterization revealed the presence of a novel 46-kb ACME and SCCmec composite island (ACME/SCCmec-CI) in ST22-MRSA-IVh isolates (n = 15). This ACME/SCCmec-CI consists of a 12-kb DNA region previously identified in ACME type II in S. epidermidis ATCC 12228, a truncated copy of the J1 region of SCCmec I and a complete SCCmec IVh element. The composite island has a novel genetic organization with ACME located within orfX and SCCmec located downstream of ACME. One pvl-positive ST22-MRSA-IVa isolate carried ACME located downstream of SCCmec IVa as previously described in ST8-MRSA-IVa. These results suggest that ACME has been acquired by ST22-MRSA-IV on two independent occasions. At least one of these instances may have involved horizontal transfer and recombination events between MRSA and CoNS. The presence of ACME may enhance dissemination of ST22-MRSA-IV, an already successful MRSA clone.Item Type
Article in PressLanguage
nullISSN
1098-6596ae974a485f413a2113503eed53cd6c53
10.1128/AAC.01756-10
Scopus Count
Collections
Related articles
- Two novel arginine catabolic mobile elements and staphylococcal chromosome cassette mec composite islands in community-acquired methicillin-resistant Staphylococcus aureus genotypes ST5-MRSA-V and ST5-MRSA-II.
- Authors: Urushibara N, Kawaguchiya M, Kobayashi N
- Issue date: 2012 Aug
- Genetic diversity of emerging Panton-Valentine leukocidine/arginine catabolic mobile element (ACME)-positive ST8 SCCmec-IVa meticillin-resistant Staphylococcus aureus (MRSA) strains and ACME-positive CC5 (ST5/ST764) MRSA strains in Northern Japan.
- Authors: Kawaguchiya M, Urushibara N, Ghosh S, Kuwahara O, Morimoto S, Ito M, Kudo K, Kobayashi N
- Issue date: 2013 Dec
- Molecular Characterization of Methicillin-Resistant Staphylococcus aureus from Outpatients in Northern Japan: Increasing Tendency of ST5/ST764 MRSA-IIa with Arginine Catabolic Mobile Element.
- Authors: Aung MS, Kawaguchiya M, Urushibara N, Sumi A, Ito M, Kudo K, Morimoto S, Hosoya S, Kobayashi N
- Issue date: 2017 Jul
- Characterization of PVL/ACME-positive methicillin-resistant Staphylococcus aureus (genotypes ST8-MRSA-IV and ST5-MRSA-II) isolated from a university hospital in Japan.
- Authors: Kawaguchiya M, Urushibara N, Yamamoto D, Yamashita T, Shinagawa M, Watanabe N, Kobayashi N
- Issue date: 2013 Feb
- An unexpected location of the arginine catabolic mobile element (ACME) in a USA300-related MRSA strain.
- Authors: Bartels MD, Hansen LH, Boye K, Sørensen SJ, Westh H
- Issue date: 2011 Jan 25