Directed evolution and targeted mutagenesis to murinize Listeria monocytogenes internalin A for enhanced infectivity in the murine oral infection model.
AffiliationAlimentary Pharmabiotic Centre & Department of Microbiology, University College Cork, Western Road, Cork, Ireland.
MeSHAmino Acid Substitution
Directed Molecular Evolution
Disease Models, Animal
Mice, Inbred BALB C
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CitationDirected evolution and targeted mutagenesis to murinize Listeria monocytogenes internalin A for enhanced infectivity in the murine oral infection model. 2010, 10:318 BMC Microbiol.
AbstractWe have used both site-directed mutagenesis and directed evolution to create variants of InlA which may inform future structure-function analyses of this protein. During the course of the study we engineered a murinized strain of L. monocytogenes EGD-e which shows reproducibly higher infectivity in the intragastric murine infection model than the wild type, but does not display enhanced entry into human cells as previously observed. This murinized L. monocytogenes strain will provide a useful tool for the analysis of the gastrointestinal phase of listeriosis.
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