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dc.contributor.authorWalsh, Fiona
dc.contributor.authorCooke, Niamh M
dc.contributor.authorSmith, Stephen G
dc.contributor.authorMoran, Gary P
dc.contributor.authorCooke, Fiona J
dc.contributor.authorIvens, Alasdair
dc.contributor.authorWain, John
dc.contributor.authorRogers, Thomas R
dc.date.accessioned2011-03-14T15:40:19Z
dc.date.available2011-03-14T15:40:19Z
dc.date.issued2010-06
dc.identifier.citationComparison of two DNA microarrays for detection of plasmid-mediated antimicrobial resistance and virulence factor genes in clinical isolates of Enterobacteriaceae and non-Enterobacteriaceae. 2010, 35 (6):593-8 Int. J. Antimicrob. Agentsen
dc.identifier.issn1872-7913
dc.identifier.pmid20356716
dc.identifier.doi10.1016/j.ijantimicag.2010.02.011
dc.identifier.urihttp://hdl.handle.net/10147/124499
dc.description.abstractA DNA microarray was developed to detect plasmid-mediated antimicrobial resistance (AR) and virulence factor (VF) genes in clinical isolates of Enterobacteriaceae and non-Enterobacteriaceae. The array was validated with the following bacterial species: Escherichiacoli (n=17); Klebsiellapneumoniae (n=3); Enterobacter spp. (n=6); Acinetobacter genospecies 3 (n=1); Acinetobacterbaumannii (n=1); Pseudomonasaeruginosa (n=2); and Stenotrophomonasmaltophilia (n=2). The AR gene profiles of these isolates were identified by polymerase chain reaction (PCR). The DNA microarray consisted of 155 and 133 AR and VF gene probes, respectively. Results were compared with the commercially available Identibac AMR-ve Array Tube. Hybridisation results indicated that there was excellent correlation between PCR and array results for AR and VF genes. Genes conferring resistance to each antibiotic class were identified by the DNA array. Unusual resistance genes were also identified, such as bla(SHV-5) in a bla(OXA-23)-positive carbapenem-resistant A. baumannii. The phylogenetic group of each E. coli isolate was verified by the array. These data demonstrate that it is possible to screen simultaneously for all important classes of mobile AR and VF genes in Enterobacteriaceae and non-Enterobacteriaceae whilst also assigning a correct phylogenetic group to E. coli isolates. Therefore, it is feasible to test clinical Gram-negative bacteria for all known AR genes and to provide important information regarding pathogenicity simultaneously.
dc.language.isoenen
dc.subject.meshBacterial Proteins
dc.subject.meshBacteriological Techniques
dc.subject.meshDrug Resistance, Bacterial
dc.subject.meshGenes, Bacterial
dc.subject.meshGram-Negative Bacteria
dc.subject.meshGram-Negative Bacterial Infections
dc.subject.meshHumans
dc.subject.meshOligonucleotide Array Sequence Analysis
dc.subject.meshSensitivity and Specificity
dc.subject.meshVirulence Factors
dc.titleComparison of two DNA microarrays for detection of plasmid-mediated antimicrobial resistance and virulence factor genes in clinical isolates of Enterobacteriaceae and non-Enterobacteriaceae.en
dc.typeArticleen
dc.contributor.departmentDepartment of Clinical Microbiology, Sir Patrick Dun Translational Research Laboratory, School of Medicine, University of Dublin, Trinity College, St James's Hospital Campus, Dublin 8, Ireland. fiona1walsh@gmail.comen
dc.identifier.journalInternational journal of antimicrobial agentsen
dc.description.provinceLeinster
html.description.abstractA DNA microarray was developed to detect plasmid-mediated antimicrobial resistance (AR) and virulence factor (VF) genes in clinical isolates of Enterobacteriaceae and non-Enterobacteriaceae. The array was validated with the following bacterial species: Escherichiacoli (n=17); Klebsiellapneumoniae (n=3); Enterobacter spp. (n=6); Acinetobacter genospecies 3 (n=1); Acinetobacterbaumannii (n=1); Pseudomonasaeruginosa (n=2); and Stenotrophomonasmaltophilia (n=2). The AR gene profiles of these isolates were identified by polymerase chain reaction (PCR). The DNA microarray consisted of 155 and 133 AR and VF gene probes, respectively. Results were compared with the commercially available Identibac AMR-ve Array Tube. Hybridisation results indicated that there was excellent correlation between PCR and array results for AR and VF genes. Genes conferring resistance to each antibiotic class were identified by the DNA array. Unusual resistance genes were also identified, such as bla(SHV-5) in a bla(OXA-23)-positive carbapenem-resistant A. baumannii. The phylogenetic group of each E. coli isolate was verified by the array. These data demonstrate that it is possible to screen simultaneously for all important classes of mobile AR and VF genes in Enterobacteriaceae and non-Enterobacteriaceae whilst also assigning a correct phylogenetic group to E. coli isolates. Therefore, it is feasible to test clinical Gram-negative bacteria for all known AR genes and to provide important information regarding pathogenicity simultaneously.


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