Purification and germination of Candida albicans and Candida dubliniensis chlamydospores cultured in liquid media.
Affiliation
Microbiology Research Unit, Division of Oral Biosciences, Dublin Dental School and Hospital, Trinity College Dublin, University of Dublin, Dublin 2, Ireland.Issue Date
2009-10MeSH
AnimalsCandida
Culture Media
Fluorescent Dyes
Macrophages
Mice
Microbial Viability
Microscopy
Microscopy, Electron, Scanning
Mycology
Spores, Fungal
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Purification and germination of Candida albicans and Candida dubliniensis chlamydospores cultured in liquid media. 2009, 9 (7):1051-60 FEMS Yeast Res.Journal
FEMS yeast researchDOI
10.1111/j.1567-1364.2009.00533.xPubMed ID
19538507Abstract
Candida albicans and Candida dubliniensis are the only Candida sp. that have been observed to produce chlamydospores. The function of these large, thick-walled cells is currently unknown. In this report, we describe the production and purification of chlamydospores from these species in defined liquid media. Staining with the fluorescent dye FUN-1 indicated that chlamydospores are metabolically active cells, but that metabolic activity is undetectable in chlamydospores that are >30 days old. However, 5-15-day-old chlamydospores could be induced to produce daughter chlamydospores, blastospores, pseudohyphae and true hyphae depending on the incubation conditions used. Chlamydospores that were preinduced to germinate were also observed to escape from murine macrophages following phagocytosis, suggesting that these structures may be viable in vivo. Mycelium-attached and purified chlamydospores rapidly lost their viability in water and when subjected to dry stress, suggesting that they are unlikely to act as long-term storage structures. Instead, our data suggest that chlamydospores represent an alternative specialized form of growth by C. albicans and C. dubliniensis.Item Type
ArticleLanguage
enISSN
1567-1364ae974a485f413a2113503eed53cd6c53
10.1111/j.1567-1364.2009.00533.x
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