Show simple item record

dc.contributor.authorO'Connor, Leanne
dc.contributor.authorCaplice, Nicole
dc.contributor.authorColeman, David C
dc.contributor.authorSullivan, Derek J
dc.contributor.authorMoran, Gary P
dc.date.accessioned2011-03-02T12:44:39Z
dc.date.available2011-03-02T12:44:39Z
dc.date.issued2010-09
dc.identifier.citationDifferential filamentation of Candida albicans and Candida dubliniensis Is governed by nutrient regulation of UME6 expression. 2010, 9 (9):1383-97 Eukaryotic Cellen
dc.identifier.issn1535-9786
dc.identifier.pmid20639413
dc.identifier.doi10.1128/EC.00042-10
dc.identifier.urihttp://hdl.handle.net/10147/123332
dc.description.abstractCandida dubliniensis is closely related to Candida albicans; however, it is responsible for fewer infections in humans and is less virulent in animal models of infection. C. dubliniensis forms fewer hyphae in vivo, and this may contribute to its reduced virulence. In this study we show that, unlike C. albicans, C. dubliniensis fails to form hyphae in yeast extract-peptone-dextrose (YPD) medium supplemented with 10% (vol/vol) fetal calf serum (YPDS medium). However, C. dubliniensis filaments in water plus 10% (vol/vol) fetal calf serum (WS), and this filamentation is inhibited by the addition of peptone and glucose. Repression of filamentation in YPDS medium could be partly overcome by preculture in synthetic Lee's medium. Unlike C. albicans, inoculation of C. dubliniensis in YPDS medium did not result in increased UME6 transcription. However, >100-fold induction of UME6 was observed when C. dubliniensis was inoculated in nutrient-poor WS medium. The addition of increasing concentrations of peptone to WS medium had a dose-dependent effect on reducing UME6 expression. Transcript profiling of C. dubliniensis hyphae in WS medium identified a starvation response involving expression of genes in the glyoxylate cycle and fatty acid oxidation. In addition, a core, shared transcriptional response with C. albicans could be identified, including expression of virulence-associated genes including SAP456, SAP7, HWP1, and SOD5. Preculture in nutrient-limiting medium enhanced adherence of C. dubliniensis, epithelial invasion, and survival following coculture with murine macrophages. In conclusion, C. albicans, unlike C. dubliniensis, appears to form hyphae in liquid medium regardless of nutrient availability, which may account for its increased capacity to cause disease in humans.
dc.language.isoenen
dc.relation.urlhttp://ec.asm.org/cgi/content/full/9/9/1383en
dc.subject.meshAnimals
dc.subject.meshCandida
dc.subject.meshCandida albicans
dc.subject.meshCandidiasis
dc.subject.meshCell Line
dc.subject.meshCulture Media
dc.subject.meshFungal Proteins
dc.subject.meshGene Expression Regulation, Developmental
dc.subject.meshGene Expression Regulation, Fungal
dc.subject.meshHumans
dc.subject.meshHyphae
dc.subject.meshMice
dc.subject.meshRepressor Proteins
dc.subject.meshVirulence
dc.titleDifferential filamentation of Candida albicans and Candida dubliniensis Is governed by nutrient regulation of UME6 expression.en
dc.contributor.departmentMicrobiology Research Unit, Division of Oral Biosciences, Dublin Dental School and Hospital, Trinity College Dublin, University of Dublin, Dublin 2, Republic of Ireland. gpmoran@dental.tcd.ie.en
dc.identifier.journalEukaryotic cellen
dc.description.provinceLeinster
html.description.abstractCandida dubliniensis is closely related to Candida albicans; however, it is responsible for fewer infections in humans and is less virulent in animal models of infection. C. dubliniensis forms fewer hyphae in vivo, and this may contribute to its reduced virulence. In this study we show that, unlike C. albicans, C. dubliniensis fails to form hyphae in yeast extract-peptone-dextrose (YPD) medium supplemented with 10% (vol/vol) fetal calf serum (YPDS medium). However, C. dubliniensis filaments in water plus 10% (vol/vol) fetal calf serum (WS), and this filamentation is inhibited by the addition of peptone and glucose. Repression of filamentation in YPDS medium could be partly overcome by preculture in synthetic Lee's medium. Unlike C. albicans, inoculation of C. dubliniensis in YPDS medium did not result in increased UME6 transcription. However, >100-fold induction of UME6 was observed when C. dubliniensis was inoculated in nutrient-poor WS medium. The addition of increasing concentrations of peptone to WS medium had a dose-dependent effect on reducing UME6 expression. Transcript profiling of C. dubliniensis hyphae in WS medium identified a starvation response involving expression of genes in the glyoxylate cycle and fatty acid oxidation. In addition, a core, shared transcriptional response with C. albicans could be identified, including expression of virulence-associated genes including SAP456, SAP7, HWP1, and SOD5. Preculture in nutrient-limiting medium enhanced adherence of C. dubliniensis, epithelial invasion, and survival following coculture with murine macrophages. In conclusion, C. albicans, unlike C. dubliniensis, appears to form hyphae in liquid medium regardless of nutrient availability, which may account for its increased capacity to cause disease in humans.


This item appears in the following Collection(s)

Show simple item record