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dc.contributor.authorSpiering, Martin J
dc.contributor.authorMoran, Gary P
dc.contributor.authorChauvel, Murielle
dc.contributor.authorMaccallum, Donna M
dc.contributor.authorHiggins, Judy
dc.contributor.authorHokamp, Karsten
dc.contributor.authorYeomans, Tim
dc.contributor.authord'Enfert, Christophe
dc.contributor.authorColeman, David C
dc.contributor.authorSullivan, Derek J
dc.date.accessioned2011-03-02T12:48:45Z
dc.date.available2011-03-02T12:48:45Z
dc.date.issued2010-02
dc.identifier.citationComparative transcript profiling of Candida albicans and Candida dubliniensis identifies SFL2, a C. albicans gene required for virulence in a reconstituted epithelial infection model. 2010, 9 (2):251-65 Eukaryotic Cellen
dc.identifier.issn1535-9786
dc.identifier.pmid20023067
dc.identifier.doi10.1128/EC.00291-09
dc.identifier.urihttp://hdl.handle.net/10147/123302
dc.description.abstractCandida albicans and Candida dubliniensis are closely related species displaying differences in virulence and genome content, therefore providing potential opportunities to identify novel C. albicans virulence genes. C. albicans gene arrays were used for comparative analysis of global gene expression in the two species in reconstituted human oral epithelium (RHE). C. albicans (SC5314) showed upregulation of hypha-specific and virulence genes within 30 min postinoculation, coinciding with rapid induction of filamentation and increased RHE damage. C. dubliniensis (CD36) showed no detectable upregulation of hypha-specific genes, grew as yeast, and caused limited RHE damage. Several genes absent or highly divergent in C. dubliniensis were upregulated in C. albicans. One such gene, SFL2 (orf19.3969), encoding a putative heat shock factor, was deleted in C. albicans. DeltaDeltasfl2 cells failed to filament under a range of hypha-inducing conditions and exhibited greatly reduced RHE damage, reversed by reintroduction of SFL2 into the DeltaDeltasfl2 strain. Moreover, SFL2 overexpression in C. albicans triggered hyphal morphogenesis. Although SFL2 deletion had no apparent effect on host survival in the murine model of systemic infection, DeltaDeltasfl2 strain-infected kidney tissues contained only yeast cells. These results suggest a role for SFL2 in morphogenesis and an indirect role in C. albicans pathogenesis in epithelial tissues.
dc.language.isoenen
dc.relation.urlhttp://ec.asm.org/cgi/content/full/9/2/251en
dc.subject.meshAnimals
dc.subject.meshCandida
dc.subject.meshCandida albicans
dc.subject.meshCandidiasis
dc.subject.meshEpithelial Cells
dc.subject.meshFemale
dc.subject.meshFungal Proteins
dc.subject.meshGene Expression Profiling
dc.subject.meshGene Expression Regulation, Fungal
dc.subject.meshGenes, Fungal
dc.subject.meshGenome, Fungal
dc.subject.meshMice
dc.subject.meshMice, Inbred BALB C
dc.subject.meshModels, Animal
dc.subject.meshTranscription, Genetic
dc.subject.meshVirulence
dc.titleComparative transcript profiling of Candida albicans and Candida dubliniensis identifies SFL2, a C. albicans gene required for virulence in a reconstituted epithelial infection model.en
dc.contributor.departmentMicrobiology Research Unit, Division of Oral Biosciences, Dublin Dental School and Hospital, University of Dublin, Trinity College, Dublin 2, Ireland.en
dc.identifier.journalEukaryotic cellen
dc.description.provinceLeinster
html.description.abstractCandida albicans and Candida dubliniensis are closely related species displaying differences in virulence and genome content, therefore providing potential opportunities to identify novel C. albicans virulence genes. C. albicans gene arrays were used for comparative analysis of global gene expression in the two species in reconstituted human oral epithelium (RHE). C. albicans (SC5314) showed upregulation of hypha-specific and virulence genes within 30 min postinoculation, coinciding with rapid induction of filamentation and increased RHE damage. C. dubliniensis (CD36) showed no detectable upregulation of hypha-specific genes, grew as yeast, and caused limited RHE damage. Several genes absent or highly divergent in C. dubliniensis were upregulated in C. albicans. One such gene, SFL2 (orf19.3969), encoding a putative heat shock factor, was deleted in C. albicans. DeltaDeltasfl2 cells failed to filament under a range of hypha-inducing conditions and exhibited greatly reduced RHE damage, reversed by reintroduction of SFL2 into the DeltaDeltasfl2 strain. Moreover, SFL2 overexpression in C. albicans triggered hyphal morphogenesis. Although SFL2 deletion had no apparent effect on host survival in the murine model of systemic infection, DeltaDeltasfl2 strain-infected kidney tissues contained only yeast cells. These results suggest a role for SFL2 in morphogenesis and an indirect role in C. albicans pathogenesis in epithelial tissues.


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