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dc.contributor.authorDobson, Lynne
dc.contributor.authorConway, Catherine
dc.contributor.authorHanley, Alan
dc.contributor.authorJohnson, Alex
dc.contributor.authorCostello, Sean
dc.contributor.authorO'Grady, Anthony
dc.contributor.authorConnolly, Yvonne
dc.contributor.authorMagee, Hilary
dc.contributor.authorO'Shea, Daniel
dc.contributor.authorJeffers, Michael
dc.contributor.authorKay, Elaine
dc.date.accessioned2010-11-16T11:22:22Z
dc.date.available2010-11-16T11:22:22Z
dc.date.issued2010-07
dc.identifier.citationImage analysis as an adjunct to manual HER-2 immunohistochemical review: a diagnostic tool to standardize interpretation. 2010, 57 (1):27-38 Histopathologyen
dc.identifier.issn1365-2559
dc.identifier.pmid20584089
dc.identifier.doi10.1111/j.1365-2559.2010.03577.x
dc.identifier.urihttp://hdl.handle.net/10147/115665
dc.description.abstractAIMS: Accurate determination of HER-2 status is critical to identify patients for whom trastuzumab treatment will be of benefit. Although the recommended primary method of evaluation is immunohistochemistry, numerous reports of variability in interpretation have raised uncertainty about the reliability of results. Recent guidelines have suggested that image analysis could be an effective tool for achieving consistent interpretation, and this study aimed to assess whether this technology has potential as a diagnostic support tool. METHODS AND RESULTS: Across a cohort of 275 cases, image analysis could accurately classify HER-2 status, with 91% agreement between computer-aided classification and the pathology review. Assessment of the continuity of membranous immunoreactivity in addition to intensity of reactivity was critical to distinguish between negative and equivocal cases and enabled image analysis to report a lower referral rate of cases for confirmatory fluorescence in situ hybridization (FISH) testing. An excellent concordance rate of 95% was observed between FISH and the automated review across 136 informative cases. CONCLUSIONS: This study has validated that image analysis can robustly and accurately evaluate HER-2 status in immunohistochemically stained tissue. Based on these findings, image analysis has great potential as a diagnostic support tool for pathologists and biomedical scientists, and may significantly improve the standardization of HER-2 testing by providing a quantitative reference method for interpretation.
dc.language.isoenen
dc.subjectBREAST CANCERen
dc.subjectIMMUNE SYSTEMen
dc.subject.meshAlgorithms
dc.subject.meshAntibodies, Monoclonal
dc.subject.meshAntineoplastic Agents
dc.subject.meshBreast Neoplasms
dc.subject.meshCohort Studies
dc.subject.meshDiagnosis, Computer-Assisted
dc.subject.meshFemale
dc.subject.meshGenes, erbB-2
dc.subject.meshHumans
dc.subject.meshImage Processing, Computer-Assisted
dc.subject.meshImmunohistochemistry
dc.subject.meshIn Situ Hybridization, Fluorescence
dc.subject.meshReceptor, erbB-2
dc.subject.meshTumor Markers, Biological
dc.titleImage analysis as an adjunct to manual HER-2 immunohistochemical review: a diagnostic tool to standardize interpretation.en
dc.typeArticleen
dc.contributor.departmentSlidePath Ltd., Swift Square, Northwood Business Park, Santry, Dublin 9, Ireland. lynne.dobson@slidepath.comen
dc.identifier.journalHistopathologyen
refterms.dateFOA2018-08-22T09:51:00Z
html.description.abstractAIMS: Accurate determination of HER-2 status is critical to identify patients for whom trastuzumab treatment will be of benefit. Although the recommended primary method of evaluation is immunohistochemistry, numerous reports of variability in interpretation have raised uncertainty about the reliability of results. Recent guidelines have suggested that image analysis could be an effective tool for achieving consistent interpretation, and this study aimed to assess whether this technology has potential as a diagnostic support tool. METHODS AND RESULTS: Across a cohort of 275 cases, image analysis could accurately classify HER-2 status, with 91% agreement between computer-aided classification and the pathology review. Assessment of the continuity of membranous immunoreactivity in addition to intensity of reactivity was critical to distinguish between negative and equivocal cases and enabled image analysis to report a lower referral rate of cases for confirmatory fluorescence in situ hybridization (FISH) testing. An excellent concordance rate of 95% was observed between FISH and the automated review across 136 informative cases. CONCLUSIONS: This study has validated that image analysis can robustly and accurately evaluate HER-2 status in immunohistochemically stained tissue. Based on these findings, image analysis has great potential as a diagnostic support tool for pathologists and biomedical scientists, and may significantly improve the standardization of HER-2 testing by providing a quantitative reference method for interpretation.


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