Neutrophil-induced transmigration of tumour cells treated with tumour-conditioned medium is facilitated by granulocyte-macrophage colony-stimulating factor.
Wu, Q D Wang, J H Bouchier-Hayes, D Redmond, H P
Wu, Q D
Wang, J H
Bouchier-Hayes, D
Redmond, H P
Advisors
Editors
Other Contributors
Date
2012-02-03T15:12:41Z
Date Submitted
Keywords
Other Subjects
Subject Mesh
Breast Neoplasms/*pathology/physiopathology
Cell Adhesion Molecules
Cell Movement
Culture Media
Cytokines/physiology
Endothelium, Vascular
Female
Granulocyte-Macrophage Colony-Stimulating Factor/*physiology
Humans
Interleukin-3/physiology
Interleukin-8/physiology
Neutrophils/*physiology
Tumor Cells, Cultured/*physiology
Cell Adhesion Molecules
Cell Movement
Culture Media
Cytokines/physiology
Endothelium, Vascular
Female
Granulocyte-Macrophage Colony-Stimulating Factor/*physiology
Humans
Interleukin-3/physiology
Interleukin-8/physiology
Neutrophils/*physiology
Tumor Cells, Cultured/*physiology
Planned Date
Start Date
Collaborators
Principal Investigators
Alternative Titles
Publisher
Abstract
OBJECTIVE: To investigate the effect of different cytokines that are present in tumour-conditioned medium on human neutrophil (PMN)-induced tumour cell transmigration. DESIGN: Laboratory study. SETTING: University hospital, Ireland. MATERIAL: Isolated human PMN and cultured human breast tumour cell line, MDA-MB-231. Interventions: Human PMN treated with either tumour-conditioned medium or different media neutralised with monoclonal antibodies (MoAb), and MDA-MB-231 cells were plated on macrovascular and microvascular endothelial monolayers in collagen-coated transwells to assess migration of tumour cells. MAIN OUTCOME MEASURES: Cytokines present in tumour-conditioned medium, PMN cytocidal function and receptor expression, and tumour cell transmigration. RESULTS: tumour-conditioned medium contained high concentrations of granulocyte-macrophage colony-stimulating factor (GM-CSF), vascular endothelial growth factor (VEGF), and interleukin 8 (IL-8), but not granulocyte colony-stimulating factor (G-CSF) and interleukin 3 (IL-3). Anti-GM-CSF MoAb significantly reduced PMN-induced transmigration of tumour cells treated with tumour-conditioned medium (p < 0.05), whereas anti-VEGF and anti-IL-8 MoAbs did not affect their migration. In addition, anti-GM-CSF MoAb, but not anti-VEGF or anti-IL-8 MoAb, reduced PMN CD11b and CD18 overexpression induced by tumour-conditioned medium (p < 0.05). CONCLUSION: These results indicate that the GM-CSF that is present in tumour-conditioned medium may be involved, at least in part, in alterations in PMN function mediated by the medium and subsequently PMN-induced transmigration of tumour cells.
Language
eng
Citation
ISSN
1102-4151 (Print)
1102-4151 (Linking)
1102-4151 (Linking)
eISSN
ISBN
DOI
10.1080/110241500750008899
PMID
10881945