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Isolation and gene expression profiling of intestinal epithelial cells: crypt isolation by calcium chelation from in vivo samples.
Balfe, Aine Lennon, Grainne Lavelle, Aonghus Docherty, Neil G Coffey, J Calvin Sheahan, Kieran Winter, Desmond C O'Connell, P Ronan
Balfe, Aine
Lennon, Grainne
Lavelle, Aonghus
Docherty, Neil G
Coffey, J Calvin
Sheahan, Kieran
Winter, Desmond C
O'Connell, P Ronan
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Advisors
Editors
Other Contributors
Departments
Date
2018
Date Submitted
Keywords
IMMUNE SYSTEM
GUT MICROBIA
GUT MICROBIA
Other Subjects
INTESTINE
Subject Mesh
Planned Date
Start Date
Collaborators
Principal Investigators
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Main Article
Adobe PDF, 1.05 MB
Alternative Titles
Abstract
The epithelial layer within the colon represents a physical barrier between the luminal contents and its underlying mucosa. It plays a pivotal role in mucosal homeostasis, and both tolerance and anti-pathogenic immune responses. Identifying signals of inflammation initiation and responses to stimuli from within the epithelial layer is critical to understanding the molecular pathways underlying disease pathology. This study validated a method to isolate and analyze epithelial populations, enabling investigations of epithelial function and response in a variety of disease setting.
Language
en
ISSN
1178-7023
eISSN
ISBN
DOI
10.2147/CEG.S145224
PMID
29391821