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Serendipitous identification of natural intergenotypic recombinants of hepatitis C in Ireland.
Moreau, Isabelle Hegarty, Susan Levis, John Sheehy, Patrick Crosbie, Orla Kenny-Walsh, Elizabeth Fanning, Liam J
Moreau, Isabelle
Hegarty, Susan
Levis, John
Sheehy, Patrick
Crosbie, Orla
Kenny-Walsh, Elizabeth
Fanning, Liam J
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Advisors
Editors
Other Contributors
Date
2006
Date Submitted
Keywords
Other Subjects
Subject Mesh
Amino Acid Sequence
Genotype
Hepacivirus
Hepatitis C
Humans
Ireland
Molecular Sequence Data
Nucleic Acid Hybridization
Recombination, Genetic
Sequence Alignment
Sequence Analysis, Protein
Viral Nonstructural Proteins
Viral Proteins
Genotype
Hepacivirus
Hepatitis C
Humans
Ireland
Molecular Sequence Data
Nucleic Acid Hybridization
Recombination, Genetic
Sequence Alignment
Sequence Analysis, Protein
Viral Nonstructural Proteins
Viral Proteins
Planned Date
Start Date
Collaborators
Principal Investigators
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17107614.pdf
Adobe PDF, 305.46 KB
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Abstract
BACKGROUND: Recombination between hepatitis C single stranded RNA viruses is a rare event. Natural viable intragenotypic and intergenotypic recombinants between 1b-1a, 1a-1c and 2k-1b, 2i-6p, respectively, have been reported. Diagnostically recombinants represent an intriguing challenge. Hepatitis C genotype is defined by interrogation of the sequence composition of the 5' untranslated region [5'UTR]. Occasionally, ambiguous specimens require further investigation of the genome, usually by interrogation of the NS5B region. The original purpose of this study was to confirm the existence of a suspected mixed genotype infection of genotypes 2 and 4 by clonal analysis at the NS5B region of the genome in two specimens from two separate individuals. This initial identification of genotype was based on analysis of the 5'UTR of the genome by reverse line probe hybridisation [RLPH]. RESULTS: The original diagnosis of a mixed genotype infection was not confirmed by clonal analysis of the NS5B region of the genome. The phylogenetic analysis indicated that both specimens were natural intergenotypic recombinant forms of HCV. The recombination was between genotypes 2k and 1b for both specimens. The recombination break point was identified as occurring within the NS2 region of the genome. CONCLUSION: The viral recombinants identified here resemble the recombinant form originally identified in Russia. The RLPH pattern observed in this study may be a signature indicative of this particular type of intergenotype recombinant of hepatitis C meriting clonal analysis of NS2.
Language
en
ISSN
1743-422X
eISSN
ISBN
DOI
10.1186/1743-422X-3-95
PMID
17107614