Bcl-x(L) expression in vivo in rheumatoid synovium.
Busteed, S Bennett, M W Molloy, C Houston, A Stone, M A Shanahan, F Molloy, M G O'Connell, J
Busteed, S
Bennett, M W
Molloy, C
Houston, A
Stone, M A
Shanahan, F
Molloy, M G
O'Connell, J
Advisors
Editors
Other Contributors
Date
2012-02-03T15:05:30Z
Date Submitted
Keywords
Other Subjects
Subject Mesh
Aged
Aging/metabolism
Arthritis, Rheumatoid/*metabolism/pathology
Blotting, Western
Endothelial Cells/metabolism
Female
Humans
Immunohistochemistry
Male
Middle Aged
Osteoarthritis/metabolism/pathology
Plasma Cells/metabolism
RNA, Messenger/metabolism
Reverse Transcriptase Polymerase Chain Reaction
Staining and Labeling
Synovial Membrane/*metabolism/pathology
Time Factors
bcl-X Protein/genetics/*metabolism
Aging/metabolism
Arthritis, Rheumatoid/*metabolism/pathology
Blotting, Western
Endothelial Cells/metabolism
Female
Humans
Immunohistochemistry
Male
Middle Aged
Osteoarthritis/metabolism/pathology
Plasma Cells/metabolism
RNA, Messenger/metabolism
Reverse Transcriptase Polymerase Chain Reaction
Staining and Labeling
Synovial Membrane/*metabolism/pathology
Time Factors
bcl-X Protein/genetics/*metabolism
Planned Date
Start Date
Collaborators
Principal Investigators
Alternative Titles
Publisher
Abstract
To examine the expression of the apoptosis regulatory protein, Bcl-x(L), in the synovium of patients with rheumatoid arthritis (RA) and osteoarthritis (OA). Immunohistochemistry for Bcl-x(L) was carried out on synovial samples from patients with RA and OA. Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot analysis were performed to qualitatively examine the expression of Bcl-x(L). Bcl-x(L) expression was detected in the lining, endothelium and inflammatory cells of both RA (n=20) and OA (n=10) samples. However, there was significantly more expression in the lining of RA synovium compared to OA (77 vs 61%, p<0.05). Many of the positive cells in the RA subsynovium were noted to be plasma cells. There was a significant correlation between Bcl-x(L) expression and the number of inflammatory cells in the subsynovium of RA and OA patients (r (s)=0.376, p<0.05, n=30). Age and disease duration did not correlate with Bcl-x(L) expression in rheumatoid patients. Bcl-x(L) may play a role in the extended survival of synoviocytes and inflammatory cells in rheumatoid synovium.
Language
eng
ISSN
0770-3198 (Print)
0770-3198 (Linking)
0770-3198 (Linking)
eISSN
ISBN
DOI
10.1007/s10067-005-0191-0
PMID
16572289