Publication

Scaffold with a natural mesh-like architecture: isolation, structural, and in vitro characterization.

Burugapalli, Krishna
; 
Thapasimuttu, Anilkumar
; 
Chan, Jeffrey C Y
; 
Yao, Li
; 
Brody, Sarah
; 
Kelly, Jack L
; 
Pandit, Abhay
Author
Burugapalli, Krishna
 Thapasimuttu, Anilkumar
 Chan, Jeffrey C Y
 Yao, Li
 Brody, Sarah
 Kelly, Jack L
 Pandit, Abhay
Advisors
Editors
Other Contributors
Departments
Department of Mechanical and Biomedical Engineering, National University of Ireland, Galway, Republic of Ireland.
Date
2007-03
Date Submitted
Keywords
Other Subjects
Subject Mesh
Animals
Connective Tissue
Cross-Linking Reagents
Elastin
Endothelium, Vascular
Extracellular Matrix
Gallbladder
Glutaral
Humans
Mice
Microscopy, Electron, Scanning
NIH 3T3 Cells
PC12 Cells
Rats
Swine
Planned Date
Start Date
Collaborators
Principal Investigators
Alternative Titles
Publisher
URI
https://hdl.handle.net/10147/143786
Abstract
An intact extracellular matrix (ECM) with a mesh-like architecture has been identified in the peri-muscular sub-serosal connective tissue (PSCT) of cholecyst (gallbladder). The PSCT layer of cholecyst wall is isolated by mechanical delamination of other layers and decellularized with a treatment with peracetic acid and ethanol solution (PES) in water to obtain the final matrix, which is referred to as cholecyst-derived ECM (CEM). CEM is cross-linked with different concentrations of glutaraldehyde (GA) to demonstrate that the susceptibility of CEM to degradation can be controlled. Quantitative and qualitative macromolecular composition assessments revealed that collagen is the primary structural component of CEM. Elastin is also present. In addition, the ultra-structural studies on CEM reveal the presence of a three-dimensional fibrous mesh-like network structure with similar nanoscale architecture on both mucosal and serosal surfaces. In vitro cell culture studies show that CEM provides a supporting structure for the attachment and proliferation of murine fibroblasts (3T3) and human umbilical vein endothelial cells (HUVEC). CEM is also shown to support the attachment and differentiation of rat adrenal pheochromocytoma cells (PC12).
Language
en
Citation
Scaffold with a natural mesh-like architecture: isolation, structural, and in vitro characterization. 2007, 8 (3):928-36 Biomacromolecules
ISSN
1525-7797
eISSN
ISBN
DOI
10.1021/bm061088x
PMID
17309297
PMCID
Sponsorships
Funding Sources
Funding Amounts
Grant Identifiers
Methodology
Duration
Ethical Approval