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Evaluation of viability and proliferative activity of human urothelial cells cultured onto xenogenic tissue-engineered extracellular matrices.
Davis, Niall F Callanan, Anthony McGuire, Barry B Flood, Hugh D McGloughlin, Tim M
Davis, Niall F
Callanan, Anthony
McGuire, Barry B
Flood, Hugh D
McGloughlin, Tim M
Advisors
Editors
Other Contributors
Date
2011-04
Date Submitted
Keywords
Other Subjects
Subject Mesh
Cell Culture Techniques
Cell Proliferation
Cell Survival
Cells, Cultured
Esterases
Ethidium
Extracellular Matrix
Humans
Immunohistochemistry
Intercalating Agents
Intestinal Mucosa
Muscle, Smooth
Oxazines
Tissue Engineering
Tissue Scaffolds
Transplantation, Heterologous
Urothelium
Xanthenes
Cell Proliferation
Cell Survival
Cells, Cultured
Esterases
Ethidium
Extracellular Matrix
Humans
Immunohistochemistry
Intercalating Agents
Intestinal Mucosa
Muscle, Smooth
Oxazines
Tissue Engineering
Tissue Scaffolds
Transplantation, Heterologous
Urothelium
Xanthenes
Planned Date
Start Date
Collaborators
Principal Investigators
Alternative Titles
Publisher
Abstract
To evaluate the viability and proliferative activity of human urothelial cells (HUCs) cultured on tissue-engineered extracellular matrix scaffolds and to assess the potential of extracellular matrixes to support the growth of HUCs in their expected in vivo urine environment.
HUCs were obtained by bladder biopsy and cultured onto the luminal and abluminal surfaces of decellularized porcine small intestinal submucosa (SIS) and porcine urinary bladder matrix (UBM). In addition, HUCs were cultured in optimal in vitro growth conditions and in their expected in vivo urine environment. The attachment, viability, and proliferative activity of HUCs were evaluated and compared using quantitative viability indicators and fluorescent markers for intracellular esterase activity and plasma membrane integrity.
The luminal and abluminal surfaces of the UBM demonstrated significantly greater HUC viability and proliferative activity compared with the luminal and abluminal surfaces of the SIS grafts (P < .0001). Culture of HUCs in a simulated in vivo urine environment significantly affected cell viability (P < .0001). Proliferative activity was immeasurable on cell-seeded scaffolds that were cultured in a urine environment after 48 hours of growth (P < .0001).
This is the first comparative report of UBM and SIS. Our results have demonstrated that UBM has significantly greater regenerative potential for HUCs compared with SIS. However, the perceived potential for extracellular matrixes in reconstructive urology might be limited by their inability to induce urothelial regeneration in a urine environment.
HUCs were obtained by bladder biopsy and cultured onto the luminal and abluminal surfaces of decellularized porcine small intestinal submucosa (SIS) and porcine urinary bladder matrix (UBM). In addition, HUCs were cultured in optimal in vitro growth conditions and in their expected in vivo urine environment. The attachment, viability, and proliferative activity of HUCs were evaluated and compared using quantitative viability indicators and fluorescent markers for intracellular esterase activity and plasma membrane integrity.
The luminal and abluminal surfaces of the UBM demonstrated significantly greater HUC viability and proliferative activity compared with the luminal and abluminal surfaces of the SIS grafts (P < .0001). Culture of HUCs in a simulated in vivo urine environment significantly affected cell viability (P < .0001). Proliferative activity was immeasurable on cell-seeded scaffolds that were cultured in a urine environment after 48 hours of growth (P < .0001).
This is the first comparative report of UBM and SIS. Our results have demonstrated that UBM has significantly greater regenerative potential for HUCs compared with SIS. However, the perceived potential for extracellular matrixes in reconstructive urology might be limited by their inability to induce urothelial regeneration in a urine environment.
Language
en
ISSN
1527-9995
eISSN
ISBN
DOI
10.1016/j.urology.2010.11.036
PMID
21256541