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Neurofilament subunit (NFL) head domain phosphorylation regulates axonal transport of neurofilaments.
Yates, Darran M Manser, Catherine De Vos, Kurt J Shaw, Christopher E McLoughlin, Declan M Miller, Christopher C J
Yates, Darran M
Manser, Catherine
De Vos, Kurt J
Shaw, Christopher E
McLoughlin, Declan M
Miller, Christopher C J
Advisors
Editors
Other Contributors
Date
2009-04
Date Submitted
Keywords
Other Subjects
Subject Mesh
Animals
Axonal Transport
Axons
Cells, Cultured
Intermediate Filaments
Mutant Proteins
Neurons
Phosphorylation
Rats
Transfection
Axonal Transport
Axons
Cells, Cultured
Intermediate Filaments
Mutant Proteins
Neurons
Phosphorylation
Rats
Transfection
Planned Date
Start Date
Collaborators
Principal Investigators
Alternative Titles
Publisher
Abstract
Neurofilaments are the intermediate filaments of neurons and are synthesised in neuronal cell bodies and then transported through axons. Neurofilament light chain (NFL) is a principal component of neurofilaments, and phosphorylation of NFL head domain is believed to regulate the assembly of neurofilaments. However, the role that NFL phosphorylation has on transport of neurofilaments is poorly understood. To address this issue, we monitored axonal transport of phosphorylation mutants of NFL. We mutated four known phosphorylation sites in NFL head domain to either preclude phosphorylation, or mimic permanent phosphorylation. Mutation to preclude phosphorylation had no effect on transport but mutation of three sites to mimic permanent phosphorylation inhibited transport. Mutation of all four sites together to mimic permanent phosphorylation proved especially potent at inhibiting transport and also disrupted neurofilament assembly. Our results suggest that NFL head domain phosphorylation is a regulator of neurofilament axonal transport.
Language
en
ISSN
1618-1298
eISSN
ISBN
DOI
10.1016/j.ejcb.2008.11.004
PMID
19147253