In silico analysis and DHPLC screening strategy identifies novel apoptotic gene targets of aberrant promoter hypermethylation in prostate cancer.
Murphy, Therese M Sullivan, Linda Lane, Caroline O'Connor, Lisa Barrett, Ciara Hollywood, Donal Lynch, Thomas Lawler, Mark Perry, Antoinette S
Murphy, Therese M
Sullivan, Linda
Lane, Caroline
O'Connor, Lisa
Barrett, Ciara
Hollywood, Donal
Lynch, Thomas
Lawler, Mark
Perry, Antoinette S
Advisors
Editors
Other Contributors
Date
2011-01-01
Date Submitted
Keywords
Apoptosis/genetics
DNA Methylation
Gene Targeting
Prostatic Neoplasms/genetics
DNA Methylation
Gene Targeting
Prostatic Neoplasms/genetics
Other Subjects
Subject Mesh
Adenovirus Infections, Human
Adult
Aged
Aged, 80 and over
Apoptosis
Cell Line, Tumor
Chromatography, High Pressure Liquid
Cohort Studies
DNA Methylation
Gene Expression Regulation, Neoplastic
Gene Targeting
Genetic Loci
Humans
Male
Middle Aged
Neoplasm Staging
Nucleic Acid Denaturation
Promoter Regions, Genetic
Prostatic Hyperplasia
Prostatic Intraepithelial Neoplasia
Prostatic Neoplasms
Adult
Aged
Aged, 80 and over
Apoptosis
Cell Line, Tumor
Chromatography, High Pressure Liquid
Cohort Studies
DNA Methylation
Gene Expression Regulation, Neoplastic
Gene Targeting
Genetic Loci
Humans
Male
Middle Aged
Neoplasm Staging
Nucleic Acid Denaturation
Promoter Regions, Genetic
Prostatic Hyperplasia
Prostatic Intraepithelial Neoplasia
Prostatic Neoplasms
Planned Date
Start Date
Collaborators
Principal Investigators
Alternative Titles
Publisher
Abstract
Aberrant DNA methylation has been implicated as a key survival mechanism in cancer, whereby promoter hypermethylation silences genes essential for many cellular processes including apoptosis. Limited data is available on the methylation profile of apoptotic genes in prostate cancer (CaP). The aim of this study was to profile methylation of apoptotic-related genes in CaP using denaturing high performance liquid chromatography (DHPLC).
Language
en
ISSN
1097-0045
eISSN
ISBN
DOI
10.1002/pros.21212
PMID
20564325