Publication

Interpretive criteria for mupirocin susceptibility testing of Staphylococcus spp. using CLSI guidelines.

Creagh, S
; 
Lucey, B
Author
Creagh, S
 Lucey, B
Advisors
Editors
Other Contributors
Departments
Department of Medical Microbiology, Cork University Hospital, Wilton, Cork,, Ireland.
Date
2012-02-03T15:17:09Z
Date Submitted
Keywords
Other Subjects
Subject Mesh
Anti-Bacterial Agents/*pharmacology
*Drug Resistance, Bacterial
Humans
Ireland
Microbial Sensitivity Tests/*methods
Multicenter Studies as Topic
Mupirocin/*pharmacology
Staphylococcal Infections/*drug therapy
Staphylococcus aureus/*drug effects/growth & development
Planned Date
Start Date
Collaborators
Principal Investigators
Alternative Titles
Publisher
URI
https://hdl.handle.net/10147/209283
Abstract
Mupirocin is an antimicrobial agent commonly used to treat staphylococcal infection or to eliminate persistent carriage. To date, interpretive criteria have not been established to define susceptibility or resistance when performing mupirocin susceptibility testing. In this evaluation, using CLSI guidelines, a total of 502 staphylococci comprising 219 methicillin-sensitive Staphylococcus aureus, 222 methicillin-resistant S. aureus and 61 coagulase-negative staphylococci are tested by broth microdilution, disc diffusion and E-test. Disc diffusion using 5 microg mupirocin discs was found to be a reliable method to distinguish susceptible and resistant strains. Minimum inhibitory concentration (MIC) determination was required to differentiate low-level and high-level resistance to mupirocin. E-test was found to be an accurate alternative to broth microdilution for the routine determination of MIC values of staphylococci to mupirocin. Broth microdilution and disc-diffusion results were plotted on a scattergram, and error rates were calculated. No errors were found using susceptibility criteria of < 4 microg/mL (MIC) and > 19 mm (zone diameter).
Language
eng
Citation
Br J Biomed Sci. 2007;64(1):1-5.
ISSN
0967-4845 (Print)
0967-4845 (Linking)
eISSN
ISBN
DOI
PMID
17444410
PMCID
Sponsorships
Funding Sources
Funding Amounts
Grant Identifiers
Methodology
Duration
Ethical Approval