Loading...
Preoperative neutrophil response as a predictive marker of clinical outcome following open heart surgery and the impact of leukocyte filtration.
Soo, Alan W Maher, Belinda M Daly, Liam Wood, Alfred E Watson, William R
Soo, Alan W
Maher, Belinda M
Daly, Liam
Wood, Alfred E
Watson, William R
Advisors
Editors
Other Contributors
Date
2010-11
Date Submitted
Keywords
Other Subjects
Subject Mesh
Aged
Antigens, CD11b
Coronary Artery Bypass
Female
Flow Cytometry
Heart Valve Prosthesis Implantation
Humans
Inflammation
L-Selectin
Leukapheresis
Leukocyte Count
Lipopolysaccharides
Male
Membrane Glycoproteins
Middle Aged
N-Formylmethionine Leucyl-Phenylalanine
Neutrophil Activation
Neutrophils
Preoperative Period
Tetradecanoylphorbol Acetate
Treatment Outcome
Antigens, CD11b
Coronary Artery Bypass
Female
Flow Cytometry
Heart Valve Prosthesis Implantation
Humans
Inflammation
L-Selectin
Leukapheresis
Leukocyte Count
Lipopolysaccharides
Male
Membrane Glycoproteins
Middle Aged
N-Formylmethionine Leucyl-Phenylalanine
Neutrophil Activation
Neutrophils
Preoperative Period
Tetradecanoylphorbol Acetate
Treatment Outcome
Planned Date
Start Date
Collaborators
Principal Investigators
Files
Loading...
20724426.pdf
Adobe PDF, 221.42 KB
Alternative Titles
Publisher
Abstract
Open heart surgery is associated with a massive systemic inflammatory response. Neutrophils, are the main mediator of this response. We hypothesised that the degree of neutrophil activation and inflammatory response to open heart surgery varies individually and correlates with clinical outcome. The aim of this study was to determine if individual clinical outcome can be predicted preoperatively through assessment of in-vitro stimulated neutrophil responses. Following that, the effects of neutrophil depletion through leukocyte filters are examined.
Neutrophil responses were assessed preoperatively (n=40) through change in neutrophil adhesion molecule [CD11b, CD62L and P Selectin Glycoprotein-1 (PSGL-1)] expression before and after in-vitro stimulation with Phorbol 12-myristate 13-acetate, PMA (1 ng/ml), lipopolysaccharide, LPS (1 μg/ml) and N-Formyl-Met-Leu-Phe, fMLP (1 ng/ml). Stimulated neutrophil responses were then correlated with postoperative clinical outcome. Patients were then randomised to leukocyte filtration (n=20) and a control group (n=20) and the effect of leukocyte filtration on neutrophil response and clinical outcome were investigated.
An individual variation in in-vitro stimulated neutrophil responses was demonstrated. Significant correlations were shown between neutrophil responses and maximum serum creatinine change, CKMB-fraction, adrenaline requirement, noradrenaline requirement, duration of adrenaline required and time to extubation. White cell count and percentage neutrophils were lower in the LD group (P=0.05). CD11b expression (P=0.005) and PSGL-1 expression (P=0.043) across leukocyte filters were also increased. However, no significant difference was detected in clinical outcome between the LD and control groups.
Preoperative neutrophil responses to in-vitro stimuli can predict clinical outcome following open heart surgery. However, leukocyte filtration did not offer significant benefit in clinical outcome in our study.
Neutrophil responses were assessed preoperatively (n=40) through change in neutrophil adhesion molecule [CD11b, CD62L and P Selectin Glycoprotein-1 (PSGL-1)] expression before and after in-vitro stimulation with Phorbol 12-myristate 13-acetate, PMA (1 ng/ml), lipopolysaccharide, LPS (1 μg/ml) and N-Formyl-Met-Leu-Phe, fMLP (1 ng/ml). Stimulated neutrophil responses were then correlated with postoperative clinical outcome. Patients were then randomised to leukocyte filtration (n=20) and a control group (n=20) and the effect of leukocyte filtration on neutrophil response and clinical outcome were investigated.
An individual variation in in-vitro stimulated neutrophil responses was demonstrated. Significant correlations were shown between neutrophil responses and maximum serum creatinine change, CKMB-fraction, adrenaline requirement, noradrenaline requirement, duration of adrenaline required and time to extubation. White cell count and percentage neutrophils were lower in the LD group (P=0.05). CD11b expression (P=0.005) and PSGL-1 expression (P=0.043) across leukocyte filters were also increased. However, no significant difference was detected in clinical outcome between the LD and control groups.
Preoperative neutrophil responses to in-vitro stimuli can predict clinical outcome following open heart surgery. However, leukocyte filtration did not offer significant benefit in clinical outcome in our study.
Language
en
ISSN
1569-9285
eISSN
ISBN
DOI
10.1510/icvts.2009.228056
PMID
20724426