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Simultaneous miRNA and mRNA transcriptome profiling of human myoblasts reveals a novel set of myogenic differentiation-associated miRNAs and their target genes
Dmitriev, Petr Barat, Ana Polesskaya, Anna O’Connell, Mary J Robert, Thomas Dessen, Philippe Walsh, Thomas A Lazar, Vladimir Turki, Ahmed Carnac, Gilles
Dmitriev, Petr
Barat, Ana
Polesskaya, Anna
O’Connell, Mary J
Robert, Thomas
Dessen, Philippe
Walsh, Thomas A
Lazar, Vladimir
Turki, Ahmed
Carnac, Gilles
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Date
2013-04-18
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Keywords
GENETICS
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1471-2164-14-265.pdf
Adobe PDF, 2.4 MB
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1471-2164-14-265-S9.PDF
Adobe PDF, 118.06 KB
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Abstract
Abstract Background miRNA profiling performed in myogenic cells and biopsies from skeletal muscles has previously identified miRNAs involved in myogenesis. Results Here, we have performed miRNA transcriptome profiling in human affinity-purified CD56+ myoblasts induced to differentiate in vitro. In total, we have identified 60 miRNAs differentially expressed during myogenic differentiation. Many were not known for being differentially expressed during myogenic differentiation. Of these, 14 (miR-23b, miR-28, miR-98, miR-103, miR-107, miR-193a, miR-210, miR-324-5p, miR-324-3p, miR-331, miR-374, miR-432, miR-502, and miR-660) were upregulated and 6 (miR-31, miR-451, miR-452, miR-565, miR-594 and miR-659) were downregulated. mRNA transcriptome profiling performed in parallel resulted in identification of 6,616 genes differentially expressed during myogenic differentiation. Conclusions This simultaneous miRNA/mRNA transcriptome profiling allowed us to predict with high accuracy target genes of myogenesis-related microRNAs and to deduce their functions.
Language
en