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MYEOV (myeloma overexpressed gene) drives colon cancer cell migration and is regulated by PGE2.
Lawlor, Garrett Doran, Peter P MacMathuna, Padraic Murray, David W
Lawlor, Garrett
Doran, Peter P
MacMathuna, Padraic
Murray, David W
Advisors
Editors
Other Contributors
Date
2010
Date Submitted
Keywords
INTESTINAL CANCER
GENETICS
GENETICS
Other Subjects
Subject Mesh
Cell Line, Tumor
Cell Movement
Cell Proliferation
Colonic Neoplasms
Dinoprostone
Humans
Proto-Oncogene Proteins
RNA, Messenger
RNA, Small Interfering
Reverse Transcriptase Polymerase Chain Reaction
Cell Movement
Cell Proliferation
Colonic Neoplasms
Dinoprostone
Humans
Proto-Oncogene Proteins
RNA, Messenger
RNA, Small Interfering
Reverse Transcriptase Polymerase Chain Reaction
Planned Date
Start Date
Collaborators
Principal Investigators
Files
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20569498.pdf
Adobe PDF, 429.07 KB
Alternative Titles
Publisher
Abstract
INTRODUCTION: We have previously reported that Myeov (MYEloma OVerexpressed gene) expression is enhanced in colorectal cancer (CRC) and that it promotes CRC cell proliferation and invasion. The role of Myeov in CRC migration is unclear. ProstaglandinE2 (PGE 2) is a known factor in promoting CRC carcinogenesis. The role of PGE 2 in modulating Myeov expression has also not been defined. AIM: To assess the role of Myeov expression in CRC cell migration and to evaluate the role of PGE 2 in Myeov bioactivity. METHODS: siRNA mediated Myeov knockdown was achieved in T84 CRC cells. Knockdown was assessed using quantitative real time PCR. The effect of knockdown on CRC cell migration was assessed using a scratch wound healing assay. Separately, T84 cells were treated with PGE 2 (0.00025 micro M, 0.1 micro M and 1 micro M) from 30 min to 3 hours and the effect on Myeov gene expression was assessed using real time PCR. RESULTS: Myeov knockdown resulted in a significant reduction in CRC cell migration, observable as early as 12 hours (P < 0.05) with a 39% reduction compared to control at 36 hours (p < 0.01). Myeov expression was enhanced after treatment with PGE 2, with the greatest effect seen at 60 mins for all 3 PGE 2 doses. This response was dose dependent with a 290%, 550% & 1,000% increase in Myeov expression for 0.00025 micro M, 0.1 micro M and 1 micro M PGE 2 respectively. CONCLUSION: In addition to promoting CRC proliferation and invasion, our findings indicate that Myeov stimulates CRC cell migration, and its expression may be PGE 2 dependant.
Language
en
ISSN
1756-9966
eISSN
ISBN
DOI
10.1186/1756-9966-29-81
PMID
20569498