Thumbnail Image
Publication

CD10/ALDH cells are the sole cisplatin-resistant component of a novel ovarian cancer stem cell hierarchy.

Ffrench, Brendan
; 
Gasch, Claudia
; 
Hokamp, Karsten
; 
Spillane, Cathy
; 
Blackshields, Gordon
; 
Mahgoub, Thamir Mahmoud
; 
Bates, Mark
; 
Kehoe, Louise
; 
Mooney, Aoibhinn
; 
Doyle, Ronan
... show 10 more
Citations
Google Scholar:
Lookup
Altmetric:
Author
Ffrench, Brendan
 Gasch, Claudia
 Hokamp, Karsten
 Spillane, Cathy
 Blackshields, Gordon
 Mahgoub, Thamir Mahmoud
 Bates, Mark
 Kehoe, Louise
 Mooney, Aoibhinn
 Doyle, Ronan
 Doyle, Brendan
 O'Donnell, Dearbhaile
 Gleeson, Noreen
 Hennessy, Bryan T
 Stordal, Britta
 O'Riain, Ciaran
 Lambkin, Helen
 O'Toole, Sharon
 O'Leary, John J
 Gallagher, Michael F
Advisors
Editors
Other Contributors
Departments
1 Department of Histopathology, Trinity College Dublin, Central Pathology Laboratory, St. James's Hospital, Dublin 8, Ireland. 2 Pathology Research Laboratory, Coombe Women and Infant's University Hospital, Dublin 8, Ireland. 3 Smurfit Institute of Genetics, Trinity College Dublin, Dublin 2, Ireland. 4 Department of Obstetrics and Gynaecology, Trinity College Dublin, Trinity Centre for Health Sciences, St. James's Hospital, Dublin 8, Ireland. 5 School of Biological Sciences, Dublin Institute of Technology, Kevin Street, Dublin, Ireland. 6 Department of Clinical Medicine, Trinity College Dublin, St. James's Hospital, Dublin 8, Ireland. 7 Department of Molecular Medicine, Royal College of Surgeons in Ireland, Dublin 2, Ireland. 8 Department of Natural Sciences, Middlesex University, Hendon, London, UK.
Date
2017-10-19
Date Submitted
Keywords
CANCER
CHEMOTHERAPY
OVARIAN CANCER
Other Subjects
Subject Mesh
Planned Date
Start Date
Collaborators
Principal Investigators
Files
Thumbnail Image
OA Article
Adobe PDF, 5.62 MB
Alternative Titles
Publisher
Springer Nature
URI
https://hdl.handle.net/10147/623921
Abstract
It is long established that tumour-initiating cancer stem cells (CSCs) possess chemoresistant properties. However, little is known of the mechanisms involved, particularly with respect to the organisation of CSCs as stem-progenitor-differentiated cell hierarchies. Here we aimed to elucidate the relationship between CSC hierarchies and chemoresistance in an ovarian cancer model. Using a single cell-based approach to CSC discovery and validation, we report a novel, four-component CSC hierarchy based around the markers cluster of differentiation 10 (CD10) and aldehyde dehydrogenase (ALDH). In a change to our understanding of CSC biology, resistance to chemotherapy drug cisplatin was found to be the sole property of CD10
Language
en
Citation
Cell Death and Disease (2017) 8, e3128; doi:10.1038/cddis.2017.379
ISSN
2041-4889
eISSN
ISBN
DOI
10.1038/cddis.2017.379
PMID
29048400
29048400
PMCID
Sponsorships
This study was primarily supported by The Emer Casey Foundation (to BF, JOL). Researchers were supported by further funding from The Irish Cancer Society (to MG, JOL), Science Foundation Ireland (to JOL) and Health Research Board (Ireland) (to JOL). We thank Professor Peter Andrews, University of Sheffield for his technical advice and support throughout the study. We also thank Dr. Eamon Breen (IMM, TCD) and Dr. Barry Moran (TBSI, TCD) for their help with flow cytometry. Funding for the flow cytometry facility (TBSI, TCD) was provided by Science Foundation Ireland (Grant 12/RI/2340(7)).
Funding Sources
Funding Amounts
Grant Identifiers
Methodology
Duration
Ethical Approval
Embedded videos