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Analysis of phenotype, genotype and serotype distribution in erythromycin-resistant group B streptococci isolated from vaginal flora in Southern Ireland.
Kiely, R A Lucey, B Cotter, L
Kiely, R A
Lucey, B
Cotter, L
Author
Advisors
Editors
Other Contributors
Date
2010-02
Date Submitted
Keywords
Other Subjects
Subject Mesh
Adolescent
Adult
Anti-Bacterial Agents
Drug Resistance, Bacterial
Erythromycin
Female
Gene Expression Regulation, Bacterial
Genotype
Humans
Ireland
Microbial Sensitivity Tests
Middle Aged
Phenotype
Serotyping
Streptococcal Infections
Streptococcus agalactiae
Vagina
Vaginal Diseases
Young Adult
Adult
Anti-Bacterial Agents
Drug Resistance, Bacterial
Erythromycin
Female
Gene Expression Regulation, Bacterial
Genotype
Humans
Ireland
Microbial Sensitivity Tests
Middle Aged
Phenotype
Serotyping
Streptococcal Infections
Streptococcus agalactiae
Vagina
Vaginal Diseases
Young Adult
Planned Date
Start Date
Collaborators
Principal Investigators
Alternative Titles
Publisher
Abstract
The screening of 2000 women of childbearing age in Cork between 2004 and 2006 produced 37 erythromycin-resistant group B streptococcus (GBS) isolates. PCR analysis was performed to determine the basis for erythromycin resistance. The ermTR gene was most frequently expressed (n = 19), followed by the ermB gene (n = 8). Four isolates harboured the mefA gene. Six isolates yielded no PCR products. Some phenotype-genotype correlation was observed. All isolates expressing the mefA gene displayed the M phenotype whilst all those expressing ermB displayed the constitutive macrolide resistance (cMLS(B)) phenotype. Of 19 isolates that expressed the ermTR gene, 16 displayed the inducible macrolide resistance (iMLS(B)) phenotype. Serotype analysis revealed that serotypes III and V predominated in these isolates. The identification of two erythromycin-resistant serotype VIII isolates among this collection represents the first reported finding of erythromycin resistance in this serotype. A single isolate was non-typable using two latex agglutination serotyping kits.
Language
en
ISSN
1469-4409
eISSN
ISBN
DOI
10.1017/S0950268809990392
PMID
19602300