Loading...
PD-1 blockade attenuates surgery-mediated immunosuppression and boosts Th1 immunity perioperatively in oesophagogastric junctional adenocarcinoma.
Davern, Maria Gaughan, Caoimhe O' Connell, Fiona Moran, Brendan Mylod, Eimear Sheppard, Andrew D Ramjit, Sinead Yun-Tong Kung, Jasmine Phelan, James J Davey, Matthew G
Davern, Maria
Gaughan, Caoimhe
O' Connell, Fiona
Moran, Brendan
Mylod, Eimear
Sheppard, Andrew D
Ramjit, Sinead
Yun-Tong Kung, Jasmine
Phelan, James J
Davey, Matthew G
Author
Davern, Maria
Gaughan, Caoimhe
O' Connell, Fiona
Moran, Brendan
Mylod, Eimear
Sheppard, Andrew D
Ramjit, Sinead
Yun-Tong Kung, Jasmine
Phelan, James J
Davey, Matthew G
Ryan, Eanna J
Butler, Christine
Quinn, Laura
Howard, Claudine
Tone, Emily
Phoenix, Eimear
Butt, Waqas T
Lynam-Lennon, Niamh
Maher, Stephen G
Ravi, Narayanasamy
Donohoe, Claire L
Reynolds, John V
Lysaght, Joanne
Donlon, Noel E
Gaughan, Caoimhe
O' Connell, Fiona
Moran, Brendan
Mylod, Eimear
Sheppard, Andrew D
Ramjit, Sinead
Yun-Tong Kung, Jasmine
Phelan, James J
Davey, Matthew G
Ryan, Eanna J
Butler, Christine
Quinn, Laura
Howard, Claudine
Tone, Emily
Phoenix, Eimear
Butt, Waqas T
Lynam-Lennon, Niamh
Maher, Stephen G
Ravi, Narayanasamy
Donohoe, Claire L
Reynolds, John V
Lysaght, Joanne
Donlon, Noel E
Advisors
Editors
Other Contributors
Departments
Date
2023-06-09
Date Submitted
Keywords
PD-1
TIGIT
Th1 immunity
Immunosuppression
nivolumab and ipilimumab
oesophageal adenocarcinoma
Perioperative
surgery
TIGIT
Th1 immunity
Immunosuppression
nivolumab and ipilimumab
oesophageal adenocarcinoma
Perioperative
surgery
Other Subjects
Subject Mesh
Planned Date
Start Date
Collaborators
Principal Investigators
Files
Loading...
fimmu-14-1150754.pdf
Adobe PDF, 5.74 MB
Alternative Titles
Publisher
Abstract
PBMCs were isolated from 11 UGI patients undergoing tumour resection on post-operative days (POD) 0, 1, 7 and 42 and expanded ex vivo using anti-CD3/28 and IL-2 for 5 days in the absence/presence of nivolumab or ipilimumab. T cells were subsequently immunophenotyped via flow cytometry to determine the frequency of T helper (Th)1-like, Th1/17-like, Th17-like and regulatory T cell (Tregs) subsets and their immune checkpoint expression profile. Lymphocyte secretions were also assessed via multiplex ELISA (IFN-γ, granzyme B, IL-17 and IL-10). The 48h cytotoxic ability of vehicle-, nivolumab- and ipilimumab-expanded PBMCs isolated on POD 0, 1, 7 and 42 against radiosensitive and radioresistant oesophageal adenocarcinoma tumour cells (OE33 P and OE33 R) was also examined using a cell counting kit-8 (CCK-8) assay to determine if surgery affected the killing ability of lymphocytes and whether the use of ICB could enhance cytotoxicity.
Language
en
Citation
ISSN
eISSN
1664-3224
ISBN
DOI
10.3389/fimmu.2023.1150754
PMID
37359545