Widespread dysregulation of MiRNAs by MYCN amplification and chromosomal imbalances in neuroblastoma: association of miRNA expression with survival.

Hdl Handle:
http://hdl.handle.net/10147/97862
Title:
Widespread dysregulation of MiRNAs by MYCN amplification and chromosomal imbalances in neuroblastoma: association of miRNA expression with survival.
Authors:
Bray, Isabella; Bryan, Kenneth; Prenter, Suzanne; Buckley, Patrick G; Foley, Niamh H; Murphy, Derek M; Alcock, Leah; Mestdagh, Pieter; Vandesompele, Jo; Speleman, Frank; London, Wendy B; McGrady, Patrick W; Higgins, Desmond G; O'Meara, Anne; O'Sullivan, Maureen; Stallings, Raymond L
Affiliation:
Department of Cancer Genetics, Royal College of Surgeons in Ireland, Dublin, Ireland.
Citation:
Widespread dysregulation of MiRNAs by MYCN amplification and chromosomal imbalances in neuroblastoma: association of miRNA expression with survival. 2009, 4 (11):e7850 PLoS ONE
Journal:
PloS one
Issue Date:
2009
URI:
http://hdl.handle.net/10147/97862
DOI:
10.1371/journal.pone.0007850
PubMed ID:
19924232
Abstract:
MiRNAs regulate gene expression at a post-transcriptional level and their dysregulation can play major roles in the pathogenesis of many different forms of cancer, including neuroblastoma, an often fatal paediatric cancer originating from precursor cells of the sympathetic nervous system. We have analyzed a set of neuroblastoma (n = 145) that is broadly representative of the genetic subtypes of this disease for miRNA expression (430 loci by stem-loop RT qPCR) and for DNA copy number alterations (array CGH) to assess miRNA involvement in disease pathogenesis. The tumors were stratified and then randomly split into a training set (n = 96) and a validation set (n = 49) for data analysis. Thirty-seven miRNAs were significantly over- or under-expressed in MYCN amplified tumors relative to MYCN single copy tumors, indicating a potential role for the MYCN transcription factor in either the direct or indirect dysregulation of these loci. In addition, we also determined that there was a highly significant correlation between miRNA expression levels and DNA copy number, indicating a role for large-scale genomic imbalances in the dysregulation of miRNA expression. In order to directly assess whether miRNA expression was predictive of clinical outcome, we used the Random Forest classifier to identify miRNAs that were most significantly associated with poor overall patient survival and developed a 15 miRNA signature that was predictive of overall survival with 72.7% sensitivity and 86.5% specificity in the validation set of tumors. We conclude that there is widespread dysregulation of miRNA expression in neuroblastoma tumors caused by both over-expression of the MYCN transcription factor and by large-scale chromosomal imbalances. MiRNA expression patterns are also predicative of clinical outcome, highlighting the potential for miRNA mediated diagnostics and therapeutics.
Language:
en
Keywords:
CANCER; GENETICS
MeSH:
Cell Line, Tumor; Chromosome Aberrations; Cluster Analysis; Cohort Studies; Gene Expression Regulation, Neoplastic; Humans; MicroRNAs; Nervous System Neoplasms; Neuroblastoma; Nuclear Proteins; Nucleic Acid Hybridization; Oncogene Proteins; Reverse Transcriptase Polymerase Chain Reaction; Sensitivity and Specificity; Sequence Analysis, DNA; Sympathetic Nervous System; Treatment Outcome
ISSN:
1932-6203

Full metadata record

DC FieldValue Language
dc.contributor.authorBray, Isabellaen
dc.contributor.authorBryan, Kennethen
dc.contributor.authorPrenter, Suzanneen
dc.contributor.authorBuckley, Patrick Gen
dc.contributor.authorFoley, Niamh Hen
dc.contributor.authorMurphy, Derek Men
dc.contributor.authorAlcock, Leahen
dc.contributor.authorMestdagh, Pieteren
dc.contributor.authorVandesompele, Joen
dc.contributor.authorSpeleman, Franken
dc.contributor.authorLondon, Wendy Ben
dc.contributor.authorMcGrady, Patrick Wen
dc.contributor.authorHiggins, Desmond Gen
dc.contributor.authorO'Meara, Anneen
dc.contributor.authorO'Sullivan, Maureenen
dc.contributor.authorStallings, Raymond Len
dc.date.accessioned2010-05-04T11:31:07Z-
dc.date.available2010-05-04T11:31:07Z-
dc.date.issued2009-
dc.identifier.citationWidespread dysregulation of MiRNAs by MYCN amplification and chromosomal imbalances in neuroblastoma: association of miRNA expression with survival. 2009, 4 (11):e7850 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid19924232-
dc.identifier.doi10.1371/journal.pone.0007850-
dc.identifier.urihttp://hdl.handle.net/10147/97862-
dc.description.abstractMiRNAs regulate gene expression at a post-transcriptional level and their dysregulation can play major roles in the pathogenesis of many different forms of cancer, including neuroblastoma, an often fatal paediatric cancer originating from precursor cells of the sympathetic nervous system. We have analyzed a set of neuroblastoma (n = 145) that is broadly representative of the genetic subtypes of this disease for miRNA expression (430 loci by stem-loop RT qPCR) and for DNA copy number alterations (array CGH) to assess miRNA involvement in disease pathogenesis. The tumors were stratified and then randomly split into a training set (n = 96) and a validation set (n = 49) for data analysis. Thirty-seven miRNAs were significantly over- or under-expressed in MYCN amplified tumors relative to MYCN single copy tumors, indicating a potential role for the MYCN transcription factor in either the direct or indirect dysregulation of these loci. In addition, we also determined that there was a highly significant correlation between miRNA expression levels and DNA copy number, indicating a role for large-scale genomic imbalances in the dysregulation of miRNA expression. In order to directly assess whether miRNA expression was predictive of clinical outcome, we used the Random Forest classifier to identify miRNAs that were most significantly associated with poor overall patient survival and developed a 15 miRNA signature that was predictive of overall survival with 72.7% sensitivity and 86.5% specificity in the validation set of tumors. We conclude that there is widespread dysregulation of miRNA expression in neuroblastoma tumors caused by both over-expression of the MYCN transcription factor and by large-scale chromosomal imbalances. MiRNA expression patterns are also predicative of clinical outcome, highlighting the potential for miRNA mediated diagnostics and therapeutics.-
dc.language.isoenen
dc.subjectCANCERen
dc.subjectGENETICSen
dc.subject.meshCell Line, Tumor-
dc.subject.meshChromosome Aberrations-
dc.subject.meshCluster Analysis-
dc.subject.meshCohort Studies-
dc.subject.meshGene Expression Regulation, Neoplastic-
dc.subject.meshHumans-
dc.subject.meshMicroRNAs-
dc.subject.meshNervous System Neoplasms-
dc.subject.meshNeuroblastoma-
dc.subject.meshNuclear Proteins-
dc.subject.meshNucleic Acid Hybridization-
dc.subject.meshOncogene Proteins-
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction-
dc.subject.meshSensitivity and Specificity-
dc.subject.meshSequence Analysis, DNA-
dc.subject.meshSympathetic Nervous System-
dc.subject.meshTreatment Outcome-
dc.titleWidespread dysregulation of MiRNAs by MYCN amplification and chromosomal imbalances in neuroblastoma: association of miRNA expression with survival.en
dc.contributor.departmentDepartment of Cancer Genetics, Royal College of Surgeons in Ireland, Dublin, Ireland.en
dc.identifier.journalPloS oneen

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